CONTINUOUS OXYGEN TENSION & PERFUSION MEASUREMENT SYSTEM

组织氧合和灌注以及它们之间的相互关系 无论是最重要的生理参数还是最难 在活体内测量。在第一阶段，我们验证了组合的可行性， 在微型混合式热敏电阻/极谱传感器中，这两种独特的 增强型热扩散探头(ETDP)的性能和最新进展 氧分压测量的进展。此第二阶段开发工作 将对所设计的原型探头/仪器系统进行细化和改进 在第一阶段，以满足这一关键需求。ETDP工具将是 扩大到包括测量组织氧分压至+0.3毫米汞柱， 再加上温度(+0.003oC)和灌注量(+10%)的测量， 具有适当的隔离以用于医疗应用。一根白金线可以 封装在热敏电阻珠的玻璃涂层中，凹入 电化学，镀金作为流动不敏感的氧 传感器。将使用水凝胶来填充极谱的凹陷 阴极以提供对蛋白质降解的免疫力。氧分压和 血流灌注将在连续运行或瞬时运行时进行测量 同时提供准确性和灵活性的模式。温度依赖关系 氧分压的测量将被确定，并且测量 将通过软件实时修正。这一集成系统将 允许检查灌流、温度和氧分压(和 它们的相互关系)在相同的小组织体积中。这一能力 是许多研究和临床研究的基础，其中包括 药理手法临床疗效的测定 循环性休克(A)的不同实验和临床状态 每年造成超过25万美国人死亡的疾病)； 局部灌流和氧分压对心脏功能的影响 室性心律失常；以及监测各种形式的 冠状动脉闭塞的溶栓治疗。商业预售 对该集成混合动力系统的评估将由我们的 整个第二阶段的技术评估和应用小组。

Tissue oxygenation and perfusion and their interrelationships are among both the most important physiologic parameters and the most difficult to measure in vivo. During Phase I, we verified the feasibility of combining, in a miniature hybrid thermistor/polarographic sensor, both the unique capability of the Enhanced Thermal Diffusion Probe (ETDP) and recent advances in oxygen tension measurements. This Phase II development effort will be refine and improve the prototype probe/instrument system designed in Phase I to meet this critical need. The ETDP instrumentation will be expanded to include the measurement of tissue oxygen tension to +0.3 mmHg, adding to the measurement of temperature (+0.003oC) and perfusion (+10%), with appropriate isolation for medical applications. A platinum wire will be encapsulated in the glass coating of a thermistor bead, recessed electrochemically, and gold plated to serve as a flow-insensitive oxygen sensor. Aqueous gels will be used to fill the recess of the polarographic cathode to provide immunity to protein degradation. Oxygen tension and perfusion will be measured in either continuous or transient operational modes to provide both accuracy and flexibility. The temperature dependence of the oxygen tension measurement will be determined, and the measurement will be corrected in real time via software. This integrated system will permit investigation of perfusion, temperature, and oxygen tension (and their interrelationships) in the same small tissue volume. This capability is fundamental to many research and clinical studies, which include determination of the effectiveness of pharmacologic manipulations in different experimental and clinical states of circulatory shock (a condition responsible for over 250,000 Americans deaths yearly); examination of the effect of local perfusion and oxygen tension on ventricular arrhythmias; and monitoring of the various forms of thrombolytic therapy for coronary artery occlusion. Pre-commercial evaluation of this integrated hybrid system will be carried out by our Technology Assessment and Applications Group throughout Phase II.