MINORITY HIGH SCHOOL STUDENT RESEARH APPRENTICE PROGRAM

德克萨斯理工大学健康科学中心有一个数字 目前从事研究的教职员工需要 有机硅复杂混合物的分离、检测和分析 蛋白质。这些调查人员中有几个使用了两个- 双向凝胶电泳法(2D-PAGE)和银凝胶电泳法 染色、放射自显影或荧光照相来实现这一点 分离和检测。其中列出的主要调查人员 一项提案正在使用2D PAGE试图检测蛋白质 它可能在类固醇合成的调节中起重要作用， 控制植入过程中涉及的事件，这些事件可能 有助于调节人体的生长、发育和代谢。 骨骼肌，可能由于以下原因而合成的蛋白质 雌激素对子宫的作用，外膜蛋白 由不同的大肠杆菌突变体合成和分泌，以及 最后，可能参与细胞识别的蛋白质 在蝌蚪尾巴回归过程中被移除。举例 生成的二维凝胶的放射自显影和荧光照片 这些研究的一部分出现在本提案的正文中。 蛋白质的分离与检测方法学 上面提到的目前正在使用中，但是， 目前还不能对目视以外的二维凝胶进行分析，因为 缺乏必要的设备。因此，这项建议是 申请资金购买计算机辅助的两台- 三维凝胶扫描设备，是必不可少的 对这里描述的蛋白质模式的成功分析。 这些扫描系统可以在商业上买到，并且能够 对蛋白质进行定量和定性分析 由2-D页面分隔。除了扫描2-D凝胶， 我们预计，该工具还将用于 一维凝胶和网点印迹的分析以及用于图像分析的 显微照片上的细胞、亚细胞细胞器和颗粒 它们正在由一个不那么复杂的、速度慢得多的 “BioQuant”成像系统目前在TTUHSC使用。

The Texas Tech University Health Sciences Center has a number of faculty who are currently engaged in research which requires the separation, detection and analysis of complex mixtures of proteins. Several of these investigators are using two- dimensional gel electrophoresis (2-D PAGE) followed by silver- staining, autoradiography or fluorography to accomplish this separation and detection. The major investigators listed in this proposal are using 2-D PAGE in an attempt to detect proteins which may by important in the regulation of steroidogenesis, the control of events involved in implantation, those which may serve in regulating the growth, development and metabolisms of skeletal muscle, proteins which may be synthesized as a result of estrogen action on the uterus, outer membrane proteins which are synthesized and secreted by various mutants of E. coli, and lastly, proteins which may be involved in the recognition of cells to be removed during tadpole tail regression. Examples of autoradiograms and fluorograms of 2-D gels generated in several of these studies appear in the text of this proposal. The methodology for the separation and detection of proteins mentioned above is currently being utilized, however, the analysis of 2-D gels other than visual is not yet possible due to a lack of the necessary equipment. Therefore, this proposal is requesting funds to purchase a computer assisted two- dimensional gel scanning device which is essential to the successful analysis of the protein patterns described herein. These scanning systems are available commercially and are able to perform both quantitative and qualitative analyses of proteins separated by 2-D PAGE. In addition to the scanning of 2-D gels, we anticipate that this instrumentation will also be used for the analysis of 1-D gels and dot-blots as well as for image analysis of cells, subcellular organelles and particles on photomicrographs which are being analysed by a less sophisticated and much slower "Bioquant" imaging system currently in use at TTUHSC.