IMAGE-PRESERVING TUNABLE FILTER FOR MICROSCOPY

一种用于细胞显微镜的液晶保像可调谐滤波器 本文提出 双折射液晶技术的最新进展 应该可以建造一个便宜的（<10 000美元），大- 孔径，可调谐滤波器比范围从400到800 nm， 毫秒，没有移动部件，带宽为10至20 nm。 有许多光学探测技术用于研究动力学， 生命的化学过程和膜运输机制 细胞 它们的功效在很大程度上取决于检测 通过监测窄带宽的激发、发射或吸收光谱 光明 随着更多探测技术的发展和系统变得越来越复杂， 复杂，自动化，多功能性，速度和方便将是 越来越重要。 国际警察工作队的液晶国际警察工作队将推动艺术 在许多这些领域。 在第一阶段，我们评估了几个单独的液晶元件， 两个组合过滤器。 在第二阶段，我们建议设计和建造 荧光显微镜成像系统的原型， 并在现场进行测试。 现场测试将比较 警察工作队对传统过滤技术的性能， 将警察工作队纳入荧光的实际方面 显微镜成像系统。

A liquid crystal image-preserving tunable filter (IPTF) for cell microscopy is proposed. Recent developments in birefringent liquid crystal technology should make it possible to construct an inexpensive (<$10,000), large- aperture, tunable filter than ranges from 400 to 800 nm in less than a millisecond with no moving parts and a 10 to 20 nm bandwidth. There are numerous optical probing techniques for studying the dynamics, the chemical processes and the membrane transport mechanisms of living cells. Their efficacy depends strongly on ability to detect shifts in excitation, emission or absorption spectra by monitoring narrow bandwidths of light. As more probing techniques are developed and systems become more complicated, automation,versatility, speed, and convenience will be increasingly important. An IPTF liquid crystal IPTF would advance the art in many of these areas. During Phase I, we evaluated several individual liquid crystal elements and two assembled filters. During Phase II, we propose to design and build prototypes for fluorescence microscope imaging systems, characterize them thoroughly, and test them at field sites. Field tests will compare the performance of the IPTF to conventional techniques of filtering and explore the practical aspects of incorporating the IPTF into fluorescence microscope imaging systems.