MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM

此应用程序申请资金以购买以下项目 仪器： 1)具有三柱合成能力的DNA合成器， 完全可编程(应用生物系统型号380B-0S)。 2)三个反应罐一次反应的多肽合成器 树脂采样器(应用生物系统430A型)。 3)高压液相色谱系统(Beckman模型 344高效液相色谱法)，可变波长检测器(165型)，以及 积分器记录仪(427型)。 这些仪器将安装在核心分子和细胞中 新近用NHLBI建设的生物实验室 资金(ICO 6 HL 35669)，并将服务于 心血管研究所生物物理学研究人员 研究所，以及肺和关节炎中心。不断增长的 分子生物学技术在生物医学中的应用 研究需要招聘新的教职员工 分子生物学专业知识和博士后的发展 几个临床科室的博士生培训计划。 每天都需要利用核苷酸合成器来 涉及其结构、功能和调控的研究 人类重要基因(载脂蛋白、收缩蛋白)的表达 蛋白质、急性期蛋白质和脂肪酸结合蛋白)。 其他蛋白质和基因目前正在鉴定中。 其中包括α受体、肾上腺素能受体、脂蛋白脂肪酶、 谷氨酸脱氢酶、钠钾ATPase。合成的 多肽还将被广泛用作蛋白水解物的抑制剂。 酶，肾素转换酶，激肽释放酶和激动素， 加压素类似物等。高效液相装置将用于 人工合成寡核苷酸的提纯与分析 多肽。对人员和人员有机构承诺 头两年操作仪器的材料。Dr。 詹姆斯·伯顿是固相多肽合成方面的专家。 Zannis，Ruiz-Opazo和Roghani将监督初始 这些仪器的安装和使用。在最初的 来自波士顿大学的支持，大部分资金支持 仪器的操作将从现有的或 新的研究拨款。费用将按比例分摊，以考虑到 考虑每个科学家的利用程度和 研究计划。类似的分摊成本的机制 对于其他核心工具，用户之间的关系已经到位， 波士顿大学。

This application request funding to purchase the following instruments: 1) A DNA synthesizer with three column synthesis capability and full programability (Applied Biosystems model 380B-0s). 2) A peptide systhesizer with three reaction vessels and a single resin sampler (Applied Biosystems model 430A). 3) High pressure liquid chromatography system (Beckman model 344HPLC), variable wave-length detector (model 165), and integrator recorder (model 427). These instruments will be installed in the core molecular and cell biology laboratories which were constructed recently with NHLBI funding (ICO 6 HL 35669) and are going to serve the needs of the investigators of the Cardiovascular Institute, the Biophysics Institute, and the Pulmonary and Arthritis Center. The growing utilization of the techniques of molecular biology in biomedical research necessitated the recruitment of new faculty with expertise in molecualr biology and the development of post doctoral training programs in several clinical departments. There is a daily need to utilize the nucleotide synthesizer for studies involving the structure, function and the regulation of expression of important human genes (apolipoproteins, contractile proteins, acute phase proteins, and fatty acid binding proteins). Other proteins and genes are currently being characterized. Among these are alpha, adrenergic receptor, lipoprotein lipase, glutamate dehydrogenase, sodium potassium ATPase. Synthetic peptides will also be used extensively as inhibitors of proteolytic enzymes, renin converting enzymes, kallikrein and kinin, vassopressin analogs, etc. The HPLC units will be used for purification and analysis of the synthetic oligonucleotides and peptides. There is an institutional commitment for personnel and materials to operate the instruments for the first two years. Dr. James Burton, an expert in solid phase peptide synthesis, and Drs. Zannis, Ruiz-Opazo, and Roghani will supervise the initial installation and operation of these instruments. After the initial support from Boston University, most of the financial support for the operation of the instruments will be obtained from existing or new research grants. The costs will be prorated to take into consideration the extent of utilization of each scientist and research program. A similar mechanism for dividing costs between users is already in place for other core instruments at Boston University.