MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM

请求提供资金，用于购买流式细胞仪/细胞分选仪 为司法部至少六组调查人员提供服务 佛罗里达大学医学院病理学。 这些调查的范围非常广泛，包括： 表面抗原、DNA含量和动力学特性分析 B）检测和 糖尿病前期和内分泌系统淋巴细胞抗原的定量 c）检测人T细胞上的抗HLA抗体， 淋巴细胞和血小板，d）细胞表面的鉴定 鉴定转移表型的标记物，和，e）鉴定和 小鼠脾B上II类分子的定量 淋巴细胞 虽然流式细胞仪/细胞分选仪已经 存在于我们的部门，并可用于这些项目， 仪器即将过时，并致力于 高优先级医院相关临床样本的分析。 的 缺乏现成的现代化和可靠的手段， 更快、更高性能的计算机正在危及当前的 这些研究人员和其他研究人员的努力， 增加了对流式细胞术和细胞分选的需求。 我们有 在这项技术和 组织和规划多用户实验室。 我们也 有合格的人员来操作和维护 仪器. 医学院的承诺和 病理学系支持我们的努力， 许多资金充足的项目需要这项技术， 确保适当的功能和适当的未来运作， 流式细胞术/分选设备。

Funds are requested to purchase a Flow Cytometer/Cell Sorter for at least six groups of investigators in the Department of Pathology of the College of Medicine of the University of Florida. The scope of these investigations is very broad and includes: a) analyses of surface antigens, DNA content and kinetic properties of human and murine lymphoma cells, b) detection and quantitation of lymphocyte antigens in prediabetes and endocrine diseases, c) detection of anti-HLA antibodies on human T- lymphocytes and platelets, d) identification of cell surface markers identifying metastic phenotypes and, e) identification and quantitation of Class II molecules on murine splenic B lymphocytes. Although a flow cytometer/cell sorter already exists in our department and is available for these projects, the instrument is reaching obsolescence and is heavily committed to analysis of high priority, hospital-related clinical samples. The lack of ready access to a modern and reliable instrument and a faster, high performance computer is jeopardizing the current efforts of these investigators and those of other researchers with increasing needs for flow cytometry and cell sorting. We have extensive experience with this technology and with the organization and planning of a multi-user laboratory. We also have competent personnel to operate and maintain the instruments. The commitments of the College of Medicine and the Department of Pathology to support our efforts, and the number of well funded projects that require this technology, insure the proper functioning and adequate future operation of the flow cytometry/sorting facility.