MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM

研究者的总体研究目标要求 这里描述的低温设施是为了定义结构 组织、关系、变更和运作机制 在生理活动中，在收缩性和收缩性的元素中， 肌肉和非肌肉细胞的细胞器和细胞支架。 我们希望在生理条件下检查这些参数 条件，或那些模仿生理状态（在 分离的大分子组装体和分子）更紧密地 比使用我们目前可用的技术更有可能， 需要用重金属固定和/或染色， 确定我们寻求的结构参数， "本土"标本。 冷冻制备：冷冻蚀刻和 将某些样本作为冷冻水化样本进行检查， 使我们能够"抓住"我们的材料在一个国家关闭，如果没有 相同的，以本地的，从而实现我们的个人 研究目标更加直接和迅速。 目前 我们是单独和共同资助：研究安排， 在骨骼和骨骼肌中， 无脊椎动物（RL）和脊椎动物（RK）的心肌， 确定生理、环境 在人行横桥的方向和厚的变化 细丝组织（RL），用于识别粗细丝上的部位 和分离的肌球蛋白分子， 以及在激活期间可能发生的其他相互作用， 肌球蛋白调节的无脊椎动物粗丝（PC，RCL）， 确定沿着粗肌丝和肌球蛋白分子的位点， 结合辅助蛋白（C、M带、H、F和X蛋白-JW），并 确定元素的结构和生物化学状态 神经突中的细胞支架和收缩器 在培养的背根伸长（或抑制该过程） 根神经节（PC）。最先进的冷冻设备 这里：巴尔泽丙烷喷射速冻和冷冻蚀刻 设备（带附件），加上Gatan标本冷冻转移 系统和低剂量包装作为我们Jeolco的修改 100CX电子显微镜将大大提高我们的研究 生产力和显着帮助我们的解释结构 与细胞和肌肉运动期间的生理事件相关。

The overall research goals of the investigators requesting the cryofacility described here is to define the structural organization, relationships, alterations and mechanisms operating during physiological activity, in elements of both the contractile apparatus and the cytoscaffold of muscle and non-muscle cells. We wish to examine these parameters under physiological conditions, or those that mimic physiological states (in the case of isolated macromolecular assemblies and molecules) more closely than is possible using the techniques presently available to us that require fixation and/or staining with heavy metals and prevent determination of the structural parameters we seek in the "native" specimens. Cryo-preparation: freeze-etching and examination of some specimens as frozen-hydrated samples will enable us to "catch" our materials in a state close, if not identical, to the native one, and thus to achieve our individual research goals much more directly and expeditiously. Currently we are singly and jointly funded: to examine the arrangement of crossbridges on the surfaces of thick filaments in skeletal and cardiac muscles of invertebrates (RL) and vertebrates (RK), to determine the influence of physiological, environmental alterations on the orientation of the crossbridges and thick filament organization (RL), to identify sites on thick filaments and isolated myosin molecules that are involved in cooperative and other interactions that may occur during activation in myosin-regulated invertebrate thick filaments (PC, RCL), to identify sites along thick filaments and myosin molecules that bind accessory proteins (C, M-band, H, F & Xproteins -JW) and to determine structural and biochemical states of the elements of the cytoscaffold and contractile apparatus involved in neurite elongation (or the inhibition of this process) in culture of dorsal root ganglion (PC). The state-of-the-art cryofacility requested here: Balzers propane jet quick-freezing and freeze-etching devices (with accessories), plus the Gatan specimen cryotransfer system and a low-dose package as a modification for our Jeolco 100CX electron microscope will greatly enhance our research productivity and significantly aid our interpretation of structural correlates of physiological events during cell and muscle motility.