INCREASED CELL CALCIUM BY OXIDATION--A POTENTIAL CAUSE OF RED CELL AGING

红细胞是心血管系统不可缺少的一部分， 氧气进入组织。这些红血球通常在循环中存活 只有大约120天的时间，它们就会被移走和销毁。虽然 许多变化发生在年轻的细胞变得“老”的时候，这些变化的原因 尚未确定，尽管大多数(如果不是全部)可以通过 细胞内钙离子增加。我们建议确定细胞 氧化及其生成的氧化产物之间的络合物， 特别是血红蛋白和细胞质可及的细胞膜 蛋白质可能通过以下途径启动红细胞衰老 细胞内钙的增加。我们还打算确定 游离离子细胞钙增加的机制是 完成了。 我们的直接目标是了解细胞衰老的机制 通过以下途径提高银行红细胞存活率的最终目标 减速或阻止红细胞衰老过程。 细胞氧化将通过治疗孤立的正常人来完成 低浓度特殊过氧化物型红细胞 一段时间。细胞内游离钙将在过氧化氢中被测量 在不同实验条件下用~(19)F- 胞内钙络合剂化合物BAPTA的核磁共振波谱。

Red blood cells are an integral part of the cardiovascular system carrying oxygen to the tissues. These red cells normally survive in circulation for only about 120 days at which time they are removed and destroyed. Although many changes occur as young cells become "old" the cause of these changes has not been identified although most, if not all, could be mediated by increased cellular calcium. We propose to determine whether cellular oxidation and the resulting complexes between oxidation products, particularly hemoglobin, and cytoplasmically accessible cell membrane proteins may be responsible for initiating red cell senescence through increases in intracellular calcium. We further intend to determine the mechanisms by which the increase in free ionic cell calcium is accomplished. Our immediate goal is to understand the mechanism of cell aging with the ultimate goal of improving the survival of banked red cells through deceleration or arrest of the red cell aging process. Cellular oxidation will be accomplished by treating isolated normal human erythrocytes with low concentrations of specific peroxides for short periods of time. Cytosolic free calcium will be measured in peroxide treated and control cells under various experimental conditions using 19F - NMR spectroscopy of the intra-cellular calcium chelator compound BAPTA.