CONFOCAL FLUORESCENCE SCANNING IMAGING SYSTEM

这是一项建立激光共聚焦荧光显微镜的建议 在华盛顿大学的星期五港湾实验室。它 将被NIH支持的几个常驻研究项目使用，如 嗯，来自美国和海外机构的其他访问调查人员。 我们建议将BioRad MRC-600共焦系统与蔡司Axiovert相结合 用于结构定性和定量成像的显微镜 复杂的断面、整装和活体材料。具体的例子 建议的工作如下：1)(A.Gordon)荧光标记的定位 皮肤肌肉纤维中的肌钙蛋白C和肌球蛋白调节轻链将 用于确定肌肉调节的分子机制 收缩。2)(J.眨眼)三磷酸肌醇诱导的研究 皮肤肌纤维中钙离子的释放。是IP3，充当 细胞内化学信使--兴奋过程中的“缺失环节” 收缩偶联？在另一个单独的项目中，发光材料的光细胞 将对蛇床子进行研究，以确定其亚细胞定位 绿色荧光蛋白。3)(A.O.D.Willows)发育跟踪 具有5-羟色胺和5-羟色胺的荧光标记神经元和轴突 多肽的抗原性将被用来回答有关组装的问题 中枢神经系统个体发育过程中神经系统的变化。这个 仪器还将支持许多其他调查人员的工作，这些调查人员 研究直接依赖于生物资源和 星期五港实验室的仪器。 FHL位于西雅图西北75英里的圣胡安岛上，是一艘海军陆战队 华盛顿大学生物实验室。它作为一种 为不同的海洋科学家提供国内和国际资源 对包括细胞和分子生物学、生理学和 生物医学。

This is a proposal to establish a laser confocal fluorescence microscope at the Friday Harbor Laboratories of the University of Washington. It will be used by several NIH supported resident research programs and, as well, by other visiting investigators from U.S. and overseas institutions. We propose combining a BioRad MRC-600 confocal system and a Zeiss Axiovert microscope for qualitative and quantitative imaging of structurally complex sections, whole mount and living material. Specific examples of work proposed are: 1) (A. Gordon) Localization of fluorescently labelled troponin C and myosin regulatory light chain in skinned muscle fibers will be used to determine molecular mechanisms of regulation of muscle contraction. 2) (J. Blinks) A study of inositol triphosphate-induced release of CA++ in skinned muscle fibers. Is IP3, acting as an intracellular chemical messenger, the "missing link" in excitation contraction coupling? In a separate project, the photocytes of luminescent cnidaria will be studied to determine the subcellular localization of the green fluorescent protein. 3) (A.O.D. Willows) Developmental tracking of fluorescence-labeled neurons and axonal processes having serotonin and peptide antigenicity will be used to answer questions about the assembly of neural subsystems during ontogeny of the central nervous system. The instrument will also support the work of many other investigators whose research is directly dependent upon the biological resources and instrumentation of the Friday Harbor Laboratories. FHL, located 75 air miles NW of Seattle on San Juan Island, is the marine biological laboratory of the University of Washington. It serves as a national and international resource for diverse marine scientists interested in topics including cell and molecular biology, physiology, and biomedicine.