LASER SCANNING CONFOCAL MICROSCOPE AND WORKSTATION

带Silicon Graphics的Biorad MRC 100激光扫描共聚焦显微镜 IRIS靛蓝工作站和选定的外围设备由一组申请 南卡罗来纳州医科大学的生物医学研究人员。 这种仪器将带来新的和强大的光学显微镜， 计算机成像技术直接影响到10个活跃或悬而未决的 NIH项目;查尔斯顿内没有其他此类设施 科学界，包括约120个NIH项目。 立即 该仪器的应用包括高分辨率 细胞内细胞骨架或信号分子的共定位， 几个实验细胞培养或器官系统和描绘 细胞外基质、粘附分子和 在发育过程中或在疾病组织中的其他分化标志物。 直接经验和试点研究表明， 计数离散的免疫定位特征，例如间隙连接或 寄生虫抗原在大体积的组织和三维 致密组织制备物中心肌纤维几何形状的表示。 在共焦显微镜和传统光方面的专业知识 显微镜和图像处理的主要用户保证充分 利用激光扫描共聚焦显微镜和 影像工作站 拟建设施的可靠运行， 确保未来能够接触到活跃的研究人员社区 过去和现在的主要用户之间的合作， 组织计划，包括技术和中央行政计划 支持.

A Biorad MRC100 laser scanning confocal microscope with Silicon Graphics IRIS Indigo workstation and selected peripherals are requested by a group of biomedical researchers at the Medical University of South Carolina. This instrument would bring new and powerful light microscopy and computer imaging technology directly to bear upon 10 active or pending NIH projects; no other such facilities exist within the Charleston scientific community, comprising some 120 NIH projects. Immediate applications of this instrumentation include high resolution colocalization of intracellular cytoskeletal or signalling molecules in several experimental cell culture or organ systems and delineation of the spatial distribution of extracellular matrix, adhesion molecules and other differentiation markers during development or in diseases tissues. Direct experience and pilot studies suggest novel applications in counting discrete immunolocalized features such as gap junctions or parasitic antigens over large volumes of tissue and three-dimensional representation of myocardial fiber geometry in dense tissue preparations. Available expertise in confocal microscopy and in conventional light microscopy and image processing among the major users assure full utilization of both the laser scanning confocal microscope and the imaging workstation. Reliable operation of the proposed facility and access to an active community of researchers in the future are assured by past and present collaborations among major users and a specific organizational plan including technical and central administrative support.