SHARED AMINO ACID ANALYZER

需要资金来购买一种现代的、最先进的氨基酸 分析仪将设在我们的生物分析中心。这台仪器将会 更换过时的Beckman MB121，将提供 灵敏、定量的氨基酸组成数据，其核心为12 用户加上广泛的其他生物医学相关科学家，请访问 华盛顿州立大学和附近的爱达荷大学。项目 将由该工具支持的活动站点研究包括 光标记和光解的收缩蛋白的分析 化学修饰和亲和标记的酶.表征 内源性多肽参与营养诱导的足抑制 摄入量；肺和卵巢中前列腺素脱氢酶的分析； 与SecB，a结合的多肽的性质研究 来自大肠杆菌的分子伴侣，合成肽的分析将是 在血管紧张素生理学和药理学研究中的应用 受体.催化的酶和突变酶的特性 萜类底物的环化反应；新化合物的表征 在植物中发现的多肽激素和病毒上的抗原部位 蛋白质；肿瘤和血清中芳香氨基酸的定量。

Funds are requested to purchase a modern, state-of-the-art amino acid analyzer to be located in our Bioanalytical Center. This instrument will replace an outdated Beckman MB121 and will provide essential, more sensitive, quantitative amino acid composition data for a core of 12 users plus a wide range of other biomedically-related scientist at Washington State University and at nearby University of Idaho. Projects to be supported by this instrument include active site studies of photolabeled and photomodified contractile proteins, analysis of chemically modified and affinity labeled enzymes; characterization of endogenous peptides involved in nutrient-induced suppression of foot intake; analysis of a prostaglandin dehydrogenase in lungs and ovaries; investigation of the properties of polypeptides bound to SecB, a molecular chaperone from E coli, analysis of synthetic peptides to be used in studying the physiology and pharmacology of angiotensin receptors; characterization of enzymes and mutant enzymes which catalyze the cyclization of terpenoid substrates; characterization of newly discovered peptide hormones in plants and antigenic sites on viral proteins; quantitation of aromatic amino acids in tumors and serum.