CANCER, LIPID, STRESS AND THYROID RESEARCH

拟议的计划旨在激励少数族裔教职员工 从事生物医学研究，为少数民族学生提供机会 获得生物医学研究经验，并使学生有兴趣追求 从事生物医学科学研究的职业。为此目的，分项目 包括： 1)黑人Graves病的分子基因分型。限制 片段长度多态(RFLP)与人类白细胞抗原(HLA) 将在患有格雷夫斯病的黑人及其家人中确定， 糖尿病患者和正常对照组。Southern印迹杂交方法将 在NICK中使用DR Alpha、DR Beta、DQ Beta和DP Beta插件 翻译将从cdna克隆获得。 2)心、肺、肾、脑的脂解酶及其底物。 该计划的目标是促进对IN的理解 体内目的的每一个(和集体)的种类繁多的内源性 酯、乙醚和神经鞘氨醇-脂类表征肾、心、肺和 脑组织中内源性脂解作用的体外检测 能力。一种精细而明确的色谱学系统 对他们的血脂进行分析将提供数据。 3)人格因素在压力相关因素的检测和控制中的作用 生理变量。这个项目涉及调查 人格因素与个体的检测和识别能力有关 主动控制与应激相关的生理变量或 心身障碍。 4)无肾毒性的铂配合物 癌细胞的化疗。该项目包括超微结构和 异丙铂联合应用的组织化学研究 卡铂和铂胸腺嘧啶治疗腹水肉瘤。剂量限制方 联合治疗的效果将受到监测。联合用药 将研究对质膜磷酸酶的影响。 5)作为抗病毒药物的杂多氟氧基金属酸盐。校长 提案研究的目标是准备、描述和探索 含钼杂多氟金属氧酸盐阴离子的抗病毒活性 W，可能还有Ru和Re作为补充物原子。抗病毒活性将会 通过(1)体外筛选作为抗病毒药物，(2)检测 样本作为抗艾滋病毒筛查过程的一部分，以及(3)研究 该化合物对一些重要的病毒酶有抑制作用。 6)有毒氨基酸的溶解、提纯和合成 亚甘氨酸A和亚甘氨酸B这个项目涉及对 两种氨基酸最终可能成为降低血液的化合物 糖。将使用一种独特的分离程序来提纯这些物质。

The proposed program is designed to motivate minority faculty members to engage in biomedical research, provide opportunities for minority students to gain biomedical research experience, and interest students in pursuing research careers in the biomedical sciences. To this end the subprojects are: 1) MOLECULAR GENOTYPING OF BLACKS WITH GRAVES' DISEASE. Restriction fragment length polymorphisms (RFLPs) and human leukocyte antigens (HLA) will be determined in blacks with Graves' Disease, their families, diabetics and normal controls. Southern blotting hybridization method will be used and DR alpha, DR beta, DQ beta and DP beta inserts used in nick translation will be obtained from cDNA clones. 2) LIPOLYTIC ENZYMES AND THEIR SUBSTRATES OF HEART, LUNG, KIDNEY AND BRAIN. The goal of this program is to contribute to the understanding of the in vivo purposes of each (and collectively) of the wide variety of endogenous ester, ether and sphingo-lipids characterizing the kidney, heart, lung and brain by an in vitro examination of their endogenous lipolytic capabilities. An elaborate and definitive system of chromatographic analysis of their lipids will provide the data. 3) PERSONALITY FACTORS IN THE DETECTION AND CONTROL OF STRESS-RELATED PHYSIOLOGICAL VARIABLES. This project involves investigation of personality factors related to the individual's ability to detect and voluntarily control physiological variables related to stress induced or psychosomatic disorders. 4) NON-NEPHROTOXIC PLATINUM COORDINATION COMPLEXES IN COMBINATION CHEMOTHERAPY OF CANCER CELLS. The project includes ultrastructural and histochemical studies of the combined application of iproplatin, carboplatin and platinum-thymine on ascites sarcoma. Dose-limiting side effects in combination therapy will be monitored. The combined drug effects on plasma membrane phosphatases will be investigated. 5) HETEROPOLYFLUOROXOMETALATES AS ANTIVIRAL AGENTS. The principal objectives of the proposal study are to prepare, characterize, and explore the antiviral activity of heteropolyfluoroxometalates anions containing Mo, W, and possibly Ru, and Re as addenda atoms. The antiviral activity will be explored by (1) in vitro screening as antiviral agents, (2) testing samples as part of the anti-HIV screening process, and (3) study the inhibitory effect of the compound on some vital viral enzymes. 6) THE SOLUTION, PURIFICATION, AND SYNTHESIS OF TOXIC AMINO ACIDS HYPOGLYCINE A AND HYPOGLYCINE B. This project involves investigation of two amino acids that could eventually serve as compounds to lower blood sugar. A unique isolation procedure will be used to purify the substances.