INCREASED CELL CALCIUM BY OXIDATION--A POTENTIAL CAUSE OF RED CELL AGING

红细胞是心血管系统的组成部分， 氧气进入组织。 这些红细胞通常在循环中存活 只有大约120天，在这个时候，他们被删除和销毁。 虽然 许多变化发生在年轻细胞变“老”的时候， 尚未确定，尽管大多数（如果不是全部）可以通过 增加细胞钙。 我们建议确定细胞是否 氧化和氧化产物之间产生的络合物， 特别是血红蛋白和细胞质可接近的细胞膜 蛋白质可能负责启动红细胞衰老， 细胞内钙离子增加。 我们还打算确定 游离离子细胞钙增加的机制是 完成了 我们的近期目标是了解细胞衰老的机制， 最终目标是通过以下方式提高库存红细胞的存活率： 延缓或阻止红细胞老化过程。 细胞氧化将通过处理分离的正常人来完成 具有低浓度特异性过氧化物的红细胞 一段时间。 将在过氧化物中测量细胞溶质游离钙 在各种实验条件下，使用19 F- 细胞内钙螯合剂化合物BAPTA的NMR光谱。

Red blood cells are an integral part of the cardiovascular system carrying oxygen to the tissues. These red cells normally survive in circulation for only about 120 days at which time they are removed and destroyed. Although many changes occur as young cells become "old" the cause of these changes has not been identified although most, if not all, could be mediated by increased cellular calcium. We propose to determine whether cellular oxidation and the resulting complexes between oxidation products, particularly hemoglobin, and cytoplasmically accessible cell membrane proteins may be responsible for initiating red cell senescence through increases in intracellular calcium. We further intend to determine the mechanisms by which the increase in free ionic cell calcium is accomplished. Our immediate goal is to understand the mechanism of cell aging with the ultimate goal of improving the survival of banked red cells through deceleration or arrest of the red cell aging process. Cellular oxidation will be accomplished by treating isolated normal human erythrocytes with low concentrations of specific peroxides for short periods of time. Cytosolic free calcium will be measured in peroxide treated and control cells under various experimental conditions using 19F - NMR spectroscopy of the intra-cellular calcium chelator compound BAPTA.