UPDATE IMAGING CORE FACILITY

此应用程序需要资金来升级成像核心 位于达拉斯的德克萨斯大学西南医学中心。影像核心 该基金成立于1988年，种子资金来自德克萨斯永久基金会 大学基金。它的四个工作站用于数字显微镜、激光 共聚焦显微镜、光漂白后荧光恢复(FRAP)和 离线图像处理被几个实验室广泛使用。 我们建议通过更换以下设备来升级数码显微镜工作站 对于过时且难以使用的图像处理器，可通过添加设备进行 为涉及活细胞的实验精确控制温度，通过添加 用于位相和高分辨率DIC显微镜的光学，通过扩展实际 利用增强型CCD摄像头和Super VHS时间流逝记录仪，并通过采集硬件和软件来做 定量光学切片和离焦反褶积。我们建议 通过添加Z轴控制器和STEP升级共焦工作站 用于定量光学切片的聚焦电机，通过将其连接到 并用Kr-Ar激光器取代现有激光器。对于 FRAP仪器我们需要一台激光剖面仪，以方便和 激光光束直径的精确测量。用于离线处理 我们需要一台视频打印机来制作出版物 高质量的黑白和彩色硬拷贝，以及光存储盘 用于高分辨率存储和显示图像的记录器。希望是这样的 有了这些补充，成像核心设施将成为 最先进的生物医学研究设施，将更好地满足 主要用户的需求。

Funds are being requested in this application to upgrade the Imaging Core Facility at U.T. Southwestern Medical Center at Dallas. The Imaging Core Facility was founded in 1988 with seed money from the Texas Permanent University Fund. Its four workstations for digital microscopy, laser confocal microscopy, fluorescence recovery after photobleaching (FRAP) and off-line image processing are extensively used by several laboratories. We are proposing to upgrade the digital microscope workstation by replacing the outdated and difficult to use image processor, by adding equipment for precise temperature control for experiments involving live cells, by adding optics for phase and high resolution DIC microscopy, by expanding the real time, low-light level capabilities with an intensified CCD camera and Super VHS time lapse recorder, and by acquiring the hardware and software to do quantitative optical sectioning and out of focus deconvolution. We propose to upgrade the confocal workstation by adding a Z-axis controller and step focus motor for quantitative optical sectioning, by connecting it to Ethernet and by replacing the existing laser with a Kr-Ar laser. For the FRAP instrument we are requesting a laser beam profiler for easy and accurate measurement of laser beam diameter. For the off-line processing workstation we are requesting a video printer for production of publication quality black and white and color hard copy, and an optical memory disk recorder for high resolution storage and display of images. It is hoped that with these additions the Imaging Core Facility will become a state-of-the-art facility for biomedical research and will better meet the needs of the major users.