EPICS-C CYTOFLUOROMETRY FOR STUDIES IN IMMUNOBIOLOGY

我们建议获得并操作EPICS-C细胞荧光计/细胞分选仪 系统与泰克4612视频复印机的目的，追求更多 我们正在进行的免疫调节淋巴细胞亚群的研究， 与移植相关的多种疾病状态和动物模型， 自身免疫性疾病和急性烧伤。 核心用户组项目a 需要80-100%的机器使用率。 我们都参与了NIH资助的研究 随着对这种仪器的需求不断增长，这无法通过 该地区现有的仪器。 我们的团队有着 合作的努力，我们大多数人现在都位于同一个 布里格姆妇女医院的实验室。 选择的工具 将允许大规模的分析和细胞分选所需的，我们的 协议. 有时，某些实验可能需要双激光器 机器，但这是在有限的基础上提供的FACS 440位于 在这家医院

We propose to acquire and operate an EPICS-C cytofluorometer/cell sorter system with a Tektronics 4612 video copier for the purpose of pursuing more efficiently our ongoing studies of immunoregulatory lymphoid subsets in a variety of disease states and animal models relating to transplantation, autoimmune disease, and acute burn injury. The core user's group project a need for 80-100% machine usage. We are all engaged in NIH funded research with a growing need for this instrumentation, and this cannot be met by existing instrumentation in the area. Our group has a history of cooperative endeavors, and most of us are now located in the same laboratory area in the Brigham and Women's Hospital. The instrument chosen will permit the largescale analysis and cell sorting needed by our protocols. On occasion, certain experiments may require a dual laser machine, but this is available on a limited basis on the FACS 440 located in this hospital.