INNER EAR ULTRASTRUCTURE AND PATHOLOGY

这是一个要求高分辨率电子显微镜与能源 分散X射线显微分析，以支持协调，超微结构 内耳的研究。 重点将放在耳蜗上 膜迷路，特别注意了解 听力障碍的原因和人工耳蜗的应用。 这套仪器将补充研究所的研究设施。 耳鼻喉科和KHRI的部门，并将创建一个独特的中心 用于耳蜗病理学和临床的超微结构研究 使用人工耳蜗。 形态分析是一种 分析和实验研究的融合， 行为、生物化学、电生理学方法来阐明 感音神经性听力损失的机制和耳蜗的作用 辅助听力受损者的假肢。 利用人力资源和 动物组织平行应导致确定的有用性 现在用于听力功能研究的动物模型。 对高分辨率电子显微镜的需求是基于 以下是积极的和待定的NIH赠款支持的具体目标：1） 检查暴露后内耳组织的超微结构特征 耳毒性药物（利尿剂、抗生素、抗利尿剂）和 强烈的噪音。 2)检测重金属，例如，抗肿瘤药 （顺铂）在内耳细胞内的分布。 3)研究 内耳屏障紧密连接的结构完整性 噪音、耳毒性物质和内耳疾病。 4)检查内耳 耳毒性药物、噪声和改变的 微稳态条件 5)识别人体内的病毒颗粒 耳标本。 6)检查病毒诱导的 耳蜗和前庭病理学。 7)检查超微结构形态 培养的上皮和内皮内耳细胞。 8)测量 培养的内皮细胞和上皮细胞的电解质 耳朵 9)检查植入假体电极的超微结构效应 耳蜗 10)检测假体电极的精神分散， 耳蜗组织

This is a request for a high resolution electron microscope with energy dispersive x-ray microanalysis to support coordinated, ultrastructural investigations of the inner ear. The focus will be on the cochlear membranous labyrinth, with specific attention given to understanding of the causes of hearing impairment and the application of cochlear prosthesis. This instrumentation package will complement the research facilities of the Department of Otorhinolaryngology and KHRI and will create a unique center for ultrastructural investigations into cochlear pathology and the clinical use of cochlear prosthesis. Morphological analysis is a point of convergence of analytical and experimental studies taking an anatomical, behavioral, biochemical, electrophysiological approach to illuminate mechanisms underlying sensorineural hearing loss and the role of cochlear prosthesis in aiding hearing impaired individuals. The use of human and animal tissue in parallel should lead to a determination of the usefulness of animal models now employed for studies of hearing function. The need for a high resolution electron microscope is based upon the following specific aims of active and pending NIH grant support: 1) Examine ultrastructural features of the inner ear tissues after exposures to ototoxic agents (diuretics, antibiotics, antineoplastic agents) and to intense noise. 2) Detect heavy metals, e.g., antineoplastic agents (cis-platinum) distribution within inner ear cells. 3) Study the structural integrity of tight junctions of inner ear barriers when stressed by noise, ototoxic agents, and inner ear disease. 4) Examine the inner ear vasculature after administration of ototoxic drugs, noise, and in altered microhomeostatic conditions. 5) Identify viral particles in human inner ear specimens. 6) Examine ultrastructural effects of viral-induced cochlear and vestibular pathology. 7) Examine ultrastructural morphology of cultured epithelial and endothelial inner ear cells. 8) Measure electrolytes in cultured epithelial and endothelial cells of the inner ear. 9) Examine ultrastructural effects of prosthetic electrodes placed in the cochlea. 10) Detect mental dispersion of prosthetic electrodes within the cochlear tissues.