PROTEIN ANALYSIS EQUIPMENT

建议购买一套专门的仪器，用于 蛋白质/多肽的纯化及其氨基酸的测定 序列。这种设备，包括一个氨基酸分析仪，气体 相控仪和制备型高效液相色谱仪， 将与已经运行的其他设备一起作为 蛋白质/核酸分析实验室(P/NAAL)，提供光谱 对用户组的分析，主要由首席调查员组成 以及加州大学欧文分校的多位同事。这些遗嘱 从完整的氨基酸序列测定到合成 寡核苷酸探针/引物。需要P/NAAL支持的项目 首席调查员实验室包括对结构和 几种依赖NAD+的脱氢酶的生物合成及其特性 大肠埃希氏菌碱性磷酸酶的突变形式及序列分析 磷酸甘油变位酶，肝脏和肠道凝集素，支持 牛精浆、豚鼠前列腺和响尾蛇的亚基 NGF和垂体成纤维细胞生长的氨基末端区域 因素。加州大学欧文分校的其他研究人员 邻近的机构需要P/NAAL支持各种项目，如 一方面，基因的结构和表达，以及对 另一方面是细胞表面受体。特别是，涉及到的项目 蛋白水解酶连接素、丙酮酸激酶同工酶、天冬酰胺的分析 合成酶、核糖体蛋白、细胞色素p-450scc、免疫球蛋白、 叶绿体醛缩酶和卤视紫红质的描述以及研究 处理蛋白质合成，细菌铁的运输。其中许多 项目还涉及基因拼接和核酸序列 决定。此外，还有相当数量的未成年人 具有同样广泛的项目种类的用户。这些东西的广度 来自主要用户和次要用户的项目强烈强调了许多 现代蛋白质化学分析的应用及其价值 提供此类服务的集中式实验室。

It is proposed to purchase a set of specialized instruments for the purification of proteins/peptides and the determination of their amino acid sequences. This equipment, which includes an amino acid analyzer, gas phase sequencer and preparative high performance liquid chromatograph, will, along with additional equipment already in operation as part of the Protein/Nucleic Acid Analysis Laboratory (P/NAAL), provide a spectrum of analyses to the users group, composed mainly of the principal investigator and various colleagues at the University of California-Irvine. These will range from full amino acid sequence determinations to the synthesis of oligonucleotide probes/primers. Projects requiring P/NAAL support from the principal investigators laboratory include studies on the structure and biosynthesis of several NAD+ dependent dehydrogenases, characterization of mutant forms of E. coli alkaline phosphatase, and the sequence analyses of phosphoglycerate mutase, liver and intestinal lectins, the supporting subunits of bovine seminal plasma, guinea pig prostate and rattlesnake NGF's, and the amino terminal region of pituitary fibroblast growth factor. Other investigators at the University of California-Irvine and neighboring institutions require P/NAAL support for projects as diverse as gene structure and expression, on the one hand, and characterization of cell surface receptors on the other. In particular, projects involving analyses of protease nexins, pyruvate kinase isozymes, asparagine synthetase, ribosomal proteins, cytochrome p-450scc, immunoglobins, chloroplast aldolases and halorhodopsin are described as well as studies dealing with protein synthesis, bacterial iron transport. Many of these projects also involve gene splicing and nucleic acid sequence determinations. In addition, there are a considerable number of minor users with an equally broad variety of projects. The breadth of these projects form both major and minor users strongly underscores the many applications of modern protein chemical analyses and the value of a centralized laboratory for providing such services.