PURCHASE OF A JASCO MODEL J-500C SPETROPOLARIMETER

所要求的分光偏振计（Jasco J-500 C）将用于各种 圆二色性研究涉及：（1） 凝血级联的各种蛋白质（凝血酶、抗凝血酶 III、因子IX、因子X）;（2）肝素与抗凝血酶的相互作用 III使用肝素衍生物和肝素八糖;（3）相互作用 蛋白质合成起始因子（eIF-2，Met-tRNA）;和（4） 甲状腺素结合球蛋白（TBG）的结构和功能研究， 甲状腺激素的主要转运蛋白。 在所有这些研究中， 非常需要一种构象敏感的工具来检测变化 在这些不同的大分子相互作用的过程中。 毫无疑问 所要求的分光偏振计将满足这一需要，并增强 这些研究人员的计划研究工作。 的存在 与凝血酶-抗凝血酶III相关的构象变化， 肝素-抗凝血酶III相互作用已被充分记录，但 这些构象变化的性质的详细方面并不 清楚 肝素在溶液中构象的研究一直是非常重要的 有限公司 与磷酸化相关的构象变化 已经假定eIF-2导致蛋白质合成的抑制，但是 缺乏直接的实验证据。 预计拟议的 抗凝血酶III-凝血酶反应的构象研究将提供 信息，以便更好地了解 抗凝血酶III和更好地了解肝素 和抗凝血酶III影响血液凝固。 详细研究 建议的肝素溶液构象应提供 关于肝素在溶液中的真实构象的非常需要的信息， 也就是说，一般的粘多糖。 拟议 对蛋白质起始因子的研究应该有助于我们 理解翻译和翻译抑制的机制 蛋白质的合成。 总的来说，这些研究的长期努力 有望提供我们了解 蛋白质-蛋白质、蛋白质-核酸和 蛋白质-粘多糖相互作用

The requested spectropolarimeter (Jasco J-500C) will be used for various circular dichroism studies dealing with: (1) the interactions of the various proteins of the blood coagulation cascade (thrombin, antithrombin III, Factor IX, Factor X); (2) interactions of heparin with antithrombin III using heparin derivatives and heparin octasaccharide; (3) interactions of protein synthesis initiation factors (eIF-2, Met-tRNA); and (4) structure and function studies of thyroxine-binding globulin (TBG), the major transport protein of the thyroid hormones. In all these studies, there is a great need for a conformation-sensitive tool to detect changes during the interactions of these various macromolecules. There is no doubt the requested spectropolarimeter will meet this need and enhance the planned research endeavors of these investigators. The existence of conformational changes associated with thrombin-antithrombin III and heparin-antithrombin III interactions have been well-documented, but the detailed aspects of the nature of these conformational changes are not clear. Studies on the conformation of heparin in solution have been very limited. The conformational changes associated with the phosphorylation of eIF-2 leading to inhibition of protein synthesis have been postulated but direct experimental evidence is lacking. It is expected that the proposed conformational studies on antithrombin III-thrombin reaction will provide information for better understanding of the complex functions of antithrombin III and better insights into the mechanism by which heparin and antithrombin III affect blood coagulation. The detailed studies proposed for the conformation of heparin in solution should provide the much needed information about the true conformation of heparin in solution, and for that matter, of the mucopolysaccharides in general. The proposed research on protein initiation factors should contribute to our understanding of the mechanism of translation and translational inhibition of protein synthesis. As a whole, the long term efforts of these studies are expected to yield information essential to our understanding of the conformational aspects of protein-protein, protein-nucleic acid and protein-mucopolysaccharide interactions.