X-RAY DIFFRACTION EQUIPMENT

这项提案的目标是升级x射线衍射 分子生物物理系使用的设备 布法罗医学基金会。 此设备是shared.by的一个主要 用户组的七个小灵通资助和两个非小灵通资助的主要 研究人员，分子生物物理系的所有成员。 共有16名专业晶体学家参与了这些项目 涉案 所申请资金的升级将为我们提供 拥有最先进的设施，包括三个 衍射仪，每个都配备了所需的附件， 研究工作的文书被指定，一个 用于蛋白质数据收集的带摄像头的旋转阳极发生器 和两个装有四个摄像头的密封管发电机， 初步摄影工作。 目前，许多组件 设备陈旧或不足以满足 实验的复杂性。 这种情况可以通过以下方式加以纠正： 更换控制两台衍射仪的计算机 使用单个microVAX II，翻新这些 仪器，更换第三批X射线发生器 具有超稳定固态发生器的衍射仪， 高稳定性低温装置的构造 分辨率工作，购买发电机和两个摄像机， 摄影工作和降低温度的装置和V形 用于蛋白质数据收集的胶片盒。

The goal of this proposal is to upgrade the x-ray diffraction equipment in use in the Molecular Biophysics Department of The Medical Foundation of Buffalo. This equipment is shared.by a major user group of seven PHS funded and two non-PHS funded principal investigators, all members of the Molecular Biophysics Department. In total 16 professional crystallographers work on the projects involved. Upgrades for which funds are requested will provide us with a state-of-the-art facility consisting of three diffractometers, each fully equipped with accessories required for research efforts for which the instruments are designated, one rotating anode generator with cameras for protein data collection and two sealed tube generators equipped with four cameras for preliminary photographic work. Presently, many of the components of the equipment are antiquated or inadequate for the level of complexity of the experiments. This situation can be corrected by the replacement of the computers that control two diffractometers with a single microVAX II, refurbishing of the goniometers of these instruments, replacement of the x-ray generator for the third diffractometer with an ultra-stable solid state generator, construction of a highly stable low temperature device for high resolution work, purchase of a generator and two cameras for photographic work and of a reduced temperature device and V-shaped film cassettes for protein data collection.