STRATOSPHERIC OZONE DEPLETION AND INCREASED DNA DAMAGE

平流层臭氧的消耗将导致太阳辐射的增加。 紫外线辐射（UVR）到达地球表面。 的类型 增加的紫外线辐射是所谓的UV-B辐射（280-320 nm），这是已知的生物损伤。 增加的 紫外线辐射的水平无疑会导致数量的增加， 人类皮肤癌。 基于先前动物的结果 致癌研究，应该可以使用DNA损伤 作为用于流行病学研究的生化标志物， 预测皮肤癌水平的增加与减少 平流层臭氧水平。 从拟议研究中获得的信息将 在决定表皮DNA水平的增加方面至关重要 在各种皮肤类型的个体中， 考虑到臭氧消耗。 具体而言，我们希望确定：1） UV-B区波长诱导DNA的有效性 皮肤类型I-V的个体的皮肤损伤， 对太阳和晒黑反应的敏感性;以及，2）使用太阳能 模拟器，以模拟与各种 臭氧消耗水平，以确定是否行动光谱 事实上，在目标1中产生的，可以用来预测增加的 臭氧对DNA的损伤程度 耗尽

Depletion of stratospheric ozone will lead to an increase in solar ultraviolet radiation (UVR) reaching the Earth's surface. The type of UVR that will increase is the so-called UV-B radiation (280-320 nm) which is known to be biologically damaging. The increased levels of UVR will undoubtedly result in an increase in the number of human skin cancers. Based on results of previous animal carcinogenesis studies, it should be possible to use DNA damage as a biochemical marker for use in epidemiological studies to predict increased levels of skin cancer associated with decreased levels of stratospheric ozone. The information obtained from the proposed research will be essential in determining increased levels of epidermal DNA damage in individuals of various skin types as a function of a given ozone depletion. Specifically, we wish to determine: 1) the effectiveness of wavelengths in the UV-B region in inducing DNA damage in the skin of individuals of skin types I-V as classified by sensitivity to the sun and tanning response; and, 2) use solar simulators to mimic UVR transmission associated with various levels of ozone depletion to determine whether the action spectra generated in aim 1 can, in fact, be used to predict increased levels of DNA damage that will occur as a result of ozone depletion.