CHARACTERIZATION OF OXYGENATED FATTY ACID METABOLITES BY CAPILLARY GC/MS

该项目的目标是开发和应用一种 灵敏和特异的气相色谱/质谱学 酒精对人脑脊液中二十烷基类化合物的测定 患者和适当的对照。这项研究是由 因为大量的文献报道表明，一段关系 存在于酒精对中枢神经系统的影响和 前列腺素的中心生产。在人类和动物中 前列腺素合成抑制剂之前的研究和给药 给药乙醇已被证明可以减弱中枢神经系统 酒精对神经系统的影响。 正常人和正常人腰椎脑脊液中前列腺素浓度 戒酒及恒河猴脑脊液中的酒精含量 所测五种前列腺素的脑脊液浓度均低于5pg/mLCSF(PGE2， PGF1、PGF2a、PGF1a、6-酮-PGF1a)。多篇文献报道 健康人体内前列腺素水平较高，例如数十至 数百pg/ml，是由于方法上的缺陷。

The objective of this project has been to develop and apply a sensitive and specific gas chromatographic / mass spectrometric assay for eicosanoids in human cerebrospinal fluid (CSF) to alcohol patients and appropriate controls. This study was initiated because numerous literature reports suggested that a relationship exists between the central nervous system effects of ethanol and the central production of prostaglandins. In both human and animal studies, administration of prostaglandin synthesis inhibitors prior to administration of ethanol has been shown to attenuate central nervous system effects of ethanol. Prostaglandin concentrations in lumbar CSF of healthy humans and abstinent the alcoholics, and in cisternal CSF of Rhesus monkeys are below 5 pg/mL CSF for the five prostaglandins measured (PGE2, PGEl, PGF2a, PGFla, 6-keto-PGFla). literature reports of much higher levels of prostaglandins in healthy humans, e.g., tens to hundreds of pg/mL, are due to methodological shortcomings.