CHARACTERIZATION OF OXYGENATED FATTY ACID METABOLITES BY CAPILLARY GC/MS

这个项目的主要目标是应用最先进的天然气 痕量分析中的色谱/质谱学方法 脂肪酸代谢产物的水平，以努力阐明分子 参与各种神经病理状态的改变。这些化合物 通常是存在于极低水平或在 如此复杂的混合物，以至于其他技术往往无法提供可靠的 结果。在这项研究的第一阶段，重点是 致力于研究人类体内前列腺素水平 脑脊液(CSF)。 初步研究表明，脑脊液中的前列腺素水平 从神经损伤患者身上提取的明显不同于 这些是正常志愿者的。这些水平通常接近或低于 报告的检出限为10-100 pg/ml的脑脊液。中描述的结果 这项研究总结了用来显著演示的方法 用于脑脊液样本分析的较低检测水平 来自不同的患者群体。特别重要的是 这些方法首次应用于脑脊液样本 取自戒酒期间的酗酒患者。

The principal objective of this project is to apply state-of-the-art gas chromatographic/mass spectrometric methodologies to the analysis of trace levels of fatty acid metabolites in an effort to elucidate the molecular alterations involved in various neuropathological states. These compounds are generally potent agents that are present in extremely low levels or in such complex mixtures that other techniques often cannot provide reliable results. During the first phase of this research, emphasis has been placed on characterizing the levels of prostaglandins in human cerebrospinal fluid (CSF). Preliminary investigations have suggested that prostaglandin levels in CSF drawn from patients with neurological impairment are markedly altered from those of normal volunteers. The levels are often near or below the reported detection limits of 10-100 pg/mL of CSF. The results described in this study summarize the methodologies used to demonstrate significantly lower levels of detection for application to the analysis of CSF samples from a variety of patient groups. Of particular importance is the application of these methodologies for the first time to samples of CSF drawn from alcoholic patients during withdrawal.