METAL IONS AND INFORMATION TRANSFER--MECHANISM OF RNA SYNTHESIS

金属离子对核酸结构和功能的影响， 确定了 现在的主要焦点是RNA的机制 在RNA聚合酶的活性位点合成。 的几何形状 在酶的活性位点的相互作用正在被探测期间， 转录过程，以确定该几何形状在转录过程中如何变化， 转录并影响其机制。 证据已经获得 这种酶通过以下方式确保复制遗传密码的准确性： 对互补性或非互补性敏感的构象变化 DNA碱基与输入碱基之间的互补性 核苷三磷酸 一个全面的忠诚机制， 已经开发出允许Mg（II）开关影响或阻止 正确的和错误的基板的结合，分别。

The effects of metal ions on nucleic acids structure and function have been determined. The primary focus now is on the mechanism of RNA synthesis at the active site of RNA polymerase. The geometry of interaction at the active site of the enzyme is being probed during the transcription process to determine how this geometry changes during transcription and affects its mechanism. Evidence has been obtained that the enzyme assures fidelity in copying the genetic code by conformation changes sensitive to complementarity or non- complementarity between DNA base and the base on the incoming nucleoside triphosphate. A comprehensive mechanism of fidelity has been developed that allows a Mg(II) switch to effect or prevent incorporation of the right and wrong substrate, respectively.