MULTIPLE ACTIVITY PATTERNS OF ACETYLCHOLINE RECEPTORS

这项提议所基于的中心观察是，在 胚胎两栖类肌肉细胞，乙酰胆碱受体通道 相似的电导有多种动力学形式。具体目标是： 1)量化控制变送器的分子速率常数 通道的不同动力学形式的结合和通道门控，2)到 确定渠道是否可以改变动力学模式，无论是作为个人还是作为 以及3)确定动力学遗传性是否以及如何 胆碱能通道与心肌细胞的发育和组装有关 神经肌肉突触。主要的实验方法是膜片钳 电生理学和单通道数据分析。这项研究是 意义重大，因为它将补充对结构-功能的研究 在胆碱能通道中的关系，它将使我们深入了解 离子通道运动的分子和细胞机制 属性是调制的，它将提供高分辨率的视图 肌肉过程中递质门控离子通道特性的变化 和突触发育。

The central observation upon which this proposal is based is that in embryonic amphibian muscle cells, acetylcholine receptor channels of similar conductance have multiple kinetic forms. The specific aims are: 1) to quantify the molecular rate constants which govern transmitter binding and channel gating in different kinetic forms of channel, 2) to determine if channels can change kinetic mode, either as individuals or as a population, and 3) to ascertain if and how kinetic hererogeneity in cholinergic channels relates to myocyte development and the assembly of the neuromuscular synapse. The major experimental method will be patch clamp electrophysiology and single-channel data analysis. The research is significant because it will compliment studies of structure-function relationships in cholinergic channels, it will give insight into the molecular and cellular mechanisms by which an ion channel's kinetic properties are modulated, and it will provide a high resolution view of changes in the properties of a transmitter-gated ion channel during muscle and synapse development.