BIOTINYLATED DNA PROBE FOR DIAGNOSIS OF CERVICAL CANCER

该项目将开发无放射性、快速简单、灵敏、 特异性和非主观性原位DNA杂交测定， 检测宫颈拭子、刮擦和活检中的特定HPV类型 标本 试验将包括在灯光下进行目视检测 在显微镜下，不会导致细胞的破坏， 形态学 这些检测将产生遗传和细胞学信息 从一个单一的宫颈标本，并将允许医生（a） 区分不同HPV类型的感染，（B） 确定患上子宫颈癌的高危病人 在细胞学异常变得明显之前，以及（c） 制定、制定和评估治疗方案 治疗HPV感染或宫颈恶性肿瘤。

This project will develop non-radioactive, rapid simple, sensitive, specific and non-subjective in situ DNA hybridization assays to detect specific HPV types in cervical swab, scrapes, and biopsy specimens. The assays will involve visual detection under a light microscope and will not result in the destruction of cellular morphology. The assays will yield both genetic and cytological information from a single cervical specimen and will allow physicians to (a) differentiate between infection with different HPV types, (b) identify patients with a high risk of developing cervical carcinoma before cytological abnormalities become apparent, and (c) develop, institute and assess therapeutic regimens for the treatment HPV infections or cervical malignancies.