DNA PROBE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS

淋球菌和衣原体感染是常见的性传播疾病 传播疾病(STD)。目前对这些疾病的诊断程序 生物体是冗长的，费力的，相对迟钝的， 不明确。一种新的以DNA为基础的诊断方法 本文对淋球菌和沙眼衣原体进行了描述。 求婚。针对每一种生物的dna探针目前 可用。DNA杂交程序使用非放射性， 生物素化的DNA探针是在我们的第一阶段研究中确定的。第二阶段 本项目将重点开发快速、人性化的 以生物素为基础的淋球菌和沙眼衣原体的非生长试验 斑点杂交、原位或夹心型DNA检测系统。这种方法 已成功应用于单纯疱疹的临床诊断 病毒。主要的重点将放在确定适当的样本上 格式、固定和杂交条件。在同期研究中， 合成寡核苷酸的使用将被开发为替代探针 技术虽然目前使用的用于从 重组资源将在短期内充足，使用 人工合成的寡核苷酸在以下方面可能提供显著的优势 成本、易用性和可重复性，适合大规模生产。

Gonococcal and Chlamydial infections are common forms of sexually transmitted diseases (STD). Current diagnostic procedures for these organisms are lengthy, laborious, and relatively insensitive and non-specific. A novel DNA-based diagnostic assay for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis is described in this proposal. DNA probes specific for each of these organisms are currently available. DNA hybridization procedures using non-radioactive, biotinylated DNA probes were determined in our Phase I studies. Phase II of this project will focus on the development of rapid, user friendly non-growth assays for N. gonorrhoeae and C. trachomatis using biotin-based dot blot, in situ, or Sandwich type DNA detection systems. This approach has been successfully applied to the clinical diagnosis of herpes simplex viruses. Major emphasis will be placed on determining appropriate sample format, fixation, and hybridization conditions. In concurrent studies, the use of synthetic oligonucleotides will be developed as an alternate probe technology. While currently used procedures for probe preparation from recombinant sources will be adequate for the near term, the use of synthetic oligonucleotides may offer significant advantages with respect to cost, ease, and reproducibility for large scale production.