DNA PROBE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS

淋球菌和衣原体感染是性传播疾病的常见形式。 传播疾病（STD）。 目前的诊断程序，这些 生物体是漫长的，费力的，相对不敏感的， 不确定 一种新的基于DNA的诊断方法，用于检测 淋病奈瑟氏菌和沙眼衣原体在这篇文章中被描述。 提议 目前，针对这些生物体中的每一种的DNA探针都是 available. 使用非放射性， 在我们的I期研究中确定了生物素化的DNA探针。 二期 本项目的重点将放在开发快速，用户友好 非生长测定N. gonorrhoeae和C.使用基于生物素的 斑点印迹、原位或三明治型DNA检测系统。 这种方法 已成功应用于单纯疱疹的临床诊断 病毒 主要重点将放在确定适当的样本 格式、固定和杂交条件。 在同期研究中， 将开发使用合成寡核苷酸作为替代探针 技术. 虽然目前使用的探针制备程序， 重组来源将是足够的，在短期内，使用 合成的寡核苷酸可以提供关于 成本、容易性和大规模生产的再现性。

Gonococcal and Chlamydial infections are common forms of sexually transmitted diseases (STD). Current diagnostic procedures for these organisms are lengthy, laborious, and relatively insensitive and non-specific. A novel DNA-based diagnostic assay for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis is described in this proposal. DNA probes specific for each of these organisms are currently available. DNA hybridization procedures using non-radioactive, biotinylated DNA probes were determined in our Phase I studies. Phase II of this project will focus on the development of rapid, user friendly non-growth assays for N. gonorrhoeae and C. trachomatis using biotin-based dot blot, in situ, or Sandwich type DNA detection systems. This approach has been successfully applied to the clinical diagnosis of herpes simplex viruses. Major emphasis will be placed on determining appropriate sample format, fixation, and hybridization conditions. In concurrent studies, the use of synthetic oligonucleotides will be developed as an alternate probe technology. While currently used procedures for probe preparation from recombinant sources will be adequate for the near term, the use of synthetic oligonucleotides may offer significant advantages with respect to cost, ease, and reproducibility for large scale production.