DEVELOP METHODS OF ANALYSIS OF HUMAN COLOSTRUM AND MILK

这个项目的总体目标是改善目前可用的 维生素K及其衍生物的分析方法学研究 人奶和初乳。这方面可用的方法有 这是一种过时的分析方法，需要大量的分析。这 该项目有可能在这一至关重要的领域取得重大进展 方法论领域。 具体地说，该项目将： 1)开发可重现且经济高效的方法 维生素K定量测定的精密度、准确度和识别力 和维生素K衍生物(维生素)，从最低剂量的母乳和 初乳； 2)证明该方法和文件的有效性和精确性 它比现有的维生素K检测方法更具优势。 母乳和初乳； 3)将此方法应用于维生素K和维生素K的定量 足月哺乳期母亲乳汁样品中的衍生物 和早产儿；以及 4)调查可能影响维生素K生物利用度的因素 在整个哺乳期的母乳和初乳中。

The general goal of this project is to improve the currently available methodology for the analysis of vitamin K and vitamin K derivatives in human milk and colostrum. The methods available in this area are antiquated and require unacceptably large volumes for analysis. This project has the potential of making significant advances in this crucial methodological area. Specifically, this project will: 1) develop reproducible and cost-effective methodology of sufficient precision, accuracy and discriminating power for quantitation of vitamin K and vitamin K derivatives (vitamers) from minimal volumes of human milk and colostrum; 2) demonstrate the validity and precision of this methodology and document its advantages over existing procedures for assessment of vitamin K in human milk and colostrum; 3) apply this methodology for the quantitation of vitamin K and vitamin K derivatives in milk samples from populations of lactating mothers of term and pre-term infants; and 4) investigate factors potentially affecting bioavailability of vitamin K in human milk and colostrum throughout the lactation period.