DEVELOP METHODS OF ANALYSIS OF HUMAN COLOSTRUM AND MILK

该项目的总体目标是改善现有的 维生素K和维生素K衍生物的分析方法 人乳和初乳。 这方面可用的方法有 过时，需要不可接受的大量分析。 这 该项目有可能在这一关键领域取得重大进展， 方法论领域。 具体而言，该项目将： 1)开发可重复和具有成本效益的方法， 维生素K定量的精密度、准确度和鉴别力 和维生素K衍生物（维生素）从最小体积的人乳， 初乳; 2)证明本方法和文件的有效性和精确性 它的优点超过现有的程序评估维生素K， 人乳和初乳; 3)应用此方法定量维生素K和维生素K 足月哺乳母亲人群乳汁样本中的衍生物 和早产儿;以及 4)研究可能影响维生素K生物利用度因素 在整个哺乳期的人乳和初乳中。

The general goal of this project is to improve the currently available methodology for the analysis of vitamin K and vitamin K derivatives in human milk and colostrum. The methods available in this area are antiquated and require unacceptably large volumes for analysis. This project has the potential of making significant advances in this crucial methodological area. Specifically, this project will: 1) develop reproducible and cost-effective methodology of sufficient precision, accuracy and discriminating power for quantitation of vitamin K and vitamin K derivatives (vitamers) from minimal volumes of human milk and colostrum; 2) demonstrate the validity and precision of this methodology and document its advantages over existing procedures for assessment of vitamin K in human milk and colostrum; 3) apply this methodology for the quantitation of vitamin K and vitamin K derivatives in milk samples from populations of lactating mothers of term and pre-term infants; and 4) investigate factors potentially affecting bioavailability of vitamin K in human milk and colostrum throughout the lactation period.