DNA PROBES FOR DIAGNOSIS OF HUMAN PAPILLOMAVIRUS TYPES

合成的短DNA探针将被开发用于诊断特定的 人乳头瘤病毒(HPV)感染类型。20-30个短DNA探针 核苷酸具有特异度高、快速等优点 反应动力学，潜在的高灵敏度和被 以低成本、大批量快速合成。他们内在的高度 专一性是一个重要的属性。现场设计是可能的 可以检测和区分任何HPV类型的杂交试验 目前已确认身份。使用较短的DNA探针，承包商将 证明了原位杂交的通用格式可以是 使用2.4M四乙基氯化铵(TEAC1)在 杂交或洗涤步骤。这种盐溶液允许杂交和 混合熔化反应仅作为探针长度的函数发生。 因此，相同长度的短探头(例如，约25 MERS)将具有 具有相同的杂交和熔化特性。此外，承包商 将演示搅动的杂交和洗涤步骤 超声波将显著减少就地手术所需的时间 现在需要在24-48小时内检测各种类型的HPV。这个 将短探头与TEAC1盐和超声波一起使用是 有望在DNA探针技术的商业化中发挥重要作用 用于临床诊断。

Synthetic short DNA probes will be developed for the diagnosis of specific types of Human Papillomavirus (HPV) infections. Short DNA probes of 20-30 nucleotides have the desirable properties of high specificity, fast reaction kinetics, potential high sensitivity and the ability to be synthesized rapidly in large amounts at low cost. Their inherent high specificity is an important attribute. It is possible to design in situ hybridization assays which can detect and discriminate any of the HPV types presently identified. With short DNA probes the contractor will demonstrate that a universal format for in situ hybridization can be developed using 2.4 M Tetraethylammoniuim chloride (TEAC1) in the hybridization or wash steps. This salt solution allows hybridization and hybrid melting reactions to occur solely as a function of probe length. Hence, short probes of the same length (e.g., ca 25 mers) will have the same hybridization and melting properties. In addition, the contractor will demonstrate that agitation of the hybridization and wash steps with ultrasound will significantly decrease the time required for the in situ detection of the various types of HPV from the 24-48 hr now required. The use of short probes in conjunction with TEAC1 salts and ultrasound is expected to be important in the commercialization of DNA probe technology for use in clinical diagnosis.