DEVELOPMENT OF METHODS FOR LARGE SCALE PEPTIDE SYNTHESIS

本项目的目标是合成并提纯0.4摩尔 或更多的多肽激素心钠素(ANP)。 这种大规模的固相多肽合成将是第一次 尝试大量合成一个超过10个氨基酸的多肽。 具体地说，这个项目的目标是开发大批量 Fmoc多肽所需的固体载体树脂 合成；制备和分析了大量的Fmoc- 酪氨酸(otBu；)和用羧基衍生树脂 心钠素的末端氨基酸，酪氨酸。

The objective of this project is to synthesize and purify 0.4 mole or more of the peptide hormone atrial natriuretic peptide (ANP). This large scale solid phase peptide synthesis would be the first attempt to mass synthesize a peptide greater than 10 amino acids. Specifically, this project is aimed at developing the large quantity of the solid support resin which is needed for teh Fmoc peptide synthesis; preparing and analyzing a large quantity of Fmoc- tyrosine (otBu; and derivatizing the resin with the carboyl terminal amino acid of ANP, tyrosine.