DEVELOPMENTAL VIROLOGY RESEARCH

艾滋病是一种病毒性疾病，临床进展与 增加病毒负荷。 将准确评估体内HIV-1水平 对监测治疗效果至关重要。 我们的实验室是第一个 开发定量培养物， HIV-1在患者的血浆和外周血单核细胞中的浓度。 此外，我们还利用这些终点稀释试验， 证明存在或不存在抗病毒药物的体内效力 剂. 在过去的两年中，HIV-1 DNA定量在临床 本实验室也实现了PCR方法对样品的扩增。 这 定量PCR技术已经允许检测的波动， 我们遇到的患者中的HIV-1载量。 我们现在建议， 这些定量的技术，使我们能够准确和定量地 在许多临床病例中检测感染性HIV-1及其核酸 标本

AIDS is a viral disease, and clinical progression is associated with increased viral burden. Accurate assessment of HIV-1 level in vivo will be critical for monitoring treatment efficacy. Our laboratory was the first to develop quantitative cultures which can determine the infectious titers of HIV-1 in the plasma and peripheral blood mononuclear cells of patients. Furthermore, we have utilized these end-point-dilution assays to demonstrate the presence or absence of in vivo efficacy of antiviral agents. In the past two years, quantitation of HIV-1 DNA in clinical samples by PCR methods has also been achieved in our laboratory. This quantitative PCR technique has permitted the detection of fluctuations in HIV-1 load in the patients encountered by us. We now propose to refine these quantitative techniques so that we can accurately and quantitatively measure infectious HIV-1 and its nucleic acids in a number of clinical specimens.