HISTOPATHOLOGY

该项目提供中央组织病理学服务， 项目在此应用中。 哥伦比亚患者的胃活检 和新奥尔良将被处理和解释的团队 病理学家如下所示。 基于先前定义的全局诊断 将为每一个案件指定标准。 此外，特别 将进行组织病理学研究，以提供更详细的 来自每个患者的信息：a）急性AS的详细方案分级 以及慢性炎症、腺颈增生、萎缩（腺损失）， 小肠和/或结肠化生和异型增生（低vs高 级）; B）核仁组成区（银NORs）; c）PCN/细胞周期蛋白 单克隆抗体; d）结缔组织染色，以评估 纤维化; e）胃蛋白酶原I和II; f）粘蛋白：PAS Alcian-Blue和HID- 阿尔新蓝染色。 每个形态参数的数值等级将 由病理学家在盲态下分配， 病人 预计定性（全球诊断）和 定量参数将提供所需的基本信息， 评估其他项目中假设的假设，即 化学预防、体内亚硝化和Helicobacter感染。 的 形态学评价也将与分子遗传学相关。 异常，提供证据，以评估遗传的时间 因果链中的伤害。

This project provides central histopathologic services for all other projects in this application. Gastric biopsies from patients in Columbia and New Orleans will be processed and interpreted by the team of pathologists shown below. A global diagnosis based on previously defined criteria will be assigned to each case. Additionally, special histopathologic studies will be carried out to provide more detailed information from each patient: a) detailed protocol gradings for acute as well as chronic inflammation, gland neck hyperplasia, atrophy (gland loss), small intestinal and/or colonic metaplasia and dysplasia (low vs high grade); b) nucleolar organizer regions (silver NORs); c) PCN/cyclin monoclonal antibody; d) connective tissue stain to evaluate the degree of fibrosis; e) pepsinogens I and II; f) mucins: PAS Alcian-Blue and HID- Alcian Blue stains. Numerical grades for each morphological parameter will be assigned by the pathologist while blinded as to the identity of the patient. It is expected that the qualitative (global diagnosis) and quantitative parameters will provide the basic information needed to evaluate the hypotheses postulated in the other projects, namely chemoprevention, in vivo nitrosation and helicobacter infection. The morphologic evaluation will also be correlated with the molecular genetic abnormalities, providing evidence to evaluate the timing of genetic injuries in the causal chain.