Cell-free synthetic biology for combinatorial biosensor design (SYNSENSO)

合成生物学领域的最新进展为下一代生物传感器提供了前所未有的机会，其特征在于可编程性，低成本以及传统传感器无法满足的灵敏度和特异性。利用合成生物学的丰富工具集，我们现在不仅可以构建更好的生物传感器，还可以构建可以感知和识别复杂分析物特征的分析工具，例如指示某种疾病状态的生物标志物面板。这些在SYNSENSO研究计划中开发的无细胞生物传感器将对许多研究和创新领域产生变革性影响，从体外诊断到健康，环境和生物过程监测。我们的目标是通过将无细胞合成生物学与分子传感器设计相结合，为生物传感领域带来变革。SYNSENSO欧洲博士网络专注于构建新型可编程配体响应元件，以及构建可以整合来自这些元件的分子信号的无细胞计算电路。这两个组件将共同组成下一代组合生物传感器，可以感知信号并计算对信号的响应。SYNSENSO旨在采用模块化的方法，将不同的响应元件与不同的计算电路组合，从而快速构建适用于新应用领域的传感器。SYNSENSO汇集了合成生物学、分子传感器设计和DNA纳米技术领域的学术和行业领导者。我们的行业合作伙伴将为该网络的十名博士候选人提供独特的生态系统。他们将与包括医学诊断专家在内的外部咨询委员会一起，对临床相关分析物进行具体的案例研究。SYNSENSO培训计划将使他们能够进行世界级的研究，并成为合成生物学和生物传感器交叉领域的学术或行业领导者。

Recent advances in the field of synthetic biology offer an unprecedented opportunity for next-generation biosensors characterized by programmability, low-cost and by a sensitivity and specificity unmet by conventional sensors. Leveraging the rich toolset of synthetic biology, we can now not only build better biosensors but also analytical tools that can sense and discern complex analyte profiles, such as a panel of biomarkers indicative of a certain disease status. These cell-free biosensors developed within the SYNSENSO research program will be transformative for many domains of research and innovation, ranging from in-vitro diagnostics to health, environmental and bioprocess monitoring. Our ambition is to provide a game change in biosensing by combining cell-free synthetic biology with molecular sensor design.The SYNSENSO European doctoral network focuses on the construction of novel programmable ligand-responsive elements and the construction of cell-free computing circuits that can integrate molecular signals from those elements. Together the two components will make up the next generation of combinatorial biosensors that can sense signals and compute a response to them. SYNSENSO aims for a modular approach where different responsive elements can be composed with different computing circuits enabling fast construction of suitable sensors for new application domains.SYNSENSO brings together academic and industry leaders from the fields of synthetic biology, molecular sensor design and DNA nanotechnology. Our industry partners will provide a unique ecosystem for ten doctoral candidates of this network. Together with the external advisory board including experts from medical diagnostics they will work on concrete case studies for clinically relevant analytes. The SYNSENSO training program will empower them to perform world-class research and become academic or industry leaders in this new emerging field at the intersection of synthetic biology and biosensors.