CONFOCAL MICROSCOPE FACILITY

我们建议建立一个共焦显微镜核心设施来服务于 西奈山医学院的不同生物医学研究人员。这个 拟建的设施将容纳一台徕卡共聚焦激光扫描显微镜 (CLSM)，具有共聚焦荧光单元和硅图 配备Voxel View图像分析软件的工作站。范围广泛的 初始用户组提出的项目(六个实验室中的八个 部门)代表了更大的潜在用户群体 共聚焦显微镜。每个研究方案都利用了共焦的 能够在固定的组织中显示荧光探针，通过 厚切片或单元层，用于执行直通聚焦系列和/或 进行三维重建。细胞生物学与解剖学系 (CB/A)已为CLSM分配部门空间 显微镜设备。机构支持包括租赁费用和 核心董事的薪水。协调中心设施主任将监督 设施的日常维护和运营。忠告 委员会包括具有光学显微镜专业知识的教员和 电子数据处理。这个设施将提供一个重要的新的 为西奈山和最直接的参与者提供的技术，7 他们中的所有人目前都得到了NIH的支持，并且都收集了数据 论证他们提议使用CLSM的可行性和必要性。 这一设施将填补西奈山的一个主要空白，那里有 基本上没有可用的共焦显微镜；单共焦 显微镜致力于其所有者的研究。

We propose to establish a Confocal Microscopy Core facility to serve diverse biomedical researchers at the Mount Sinai School of Medicine. The proposed facility would house a Leica confocal laser scanning microscope (CLSM) with a confocal fluorescence unit, and a Silicon Graphics workstation with Voxel View image analysis software. The broad range of projects proposed by the initial user group (eight laboratories in six departments) is representative of a larger group of potential users of confocal microscopy. Each research proposal utilizes the confocal's ability to visualize fluorescent probes in fixed tissue, to focus through thick sections or cell layers, to execute a through focus series and/or to perform 3-D reconstruction. The Cell Biology and Anatomy Department (CB/A) has allocated departmental space for the CLSM within its microscopy facility. Institutional support includes leasing expenses and salary for the Core director. The Confocal Facility Director will oversee the day-to-day maintenance and operation of the facility. The advisory committee includes faculty who have expertise in light microscopy and electronic data processing. This facility will provide a significant new technology for Mount Sinai and most directly for the participants, seven of whom have current NIH support and all of whom have collected data demonstrating feasibility and need for their proposed uses of the CLSM. This facility will fill a major void at Mount Sinai where there is essentially no confocal microscope available; the single confocal microscope is dedicated to the research of its owner.