MOLECULAR BIOLOGY OF NEURAL FUNCTION

下面的摘要描述了五个项目， 在分子水平上研究一些分子的作用 包括，驱动蛋白轻链，rab 3，p67，一种新的神经元激活剂， Cdk 5、调节性神经肽buccalin和myomodulin以及酸性 calponin钙调蛋白，play玩in neural神经function功能. 驱动蛋白轻链、rab 3和p67 是直接或间接影响突触囊泡的蛋白质 运输或融合。我们目前正试图解决蛋白质 通过同源重组进行突变和敲除分析 与驱动蛋白轻链和p67或通过蛋白质表达， 在Rab 3的情况下进行显微注射。 我们之前已经描述了 海兔Buccalin和Myomodulin的肽家族 目前正在研究他们如何共同贡献 到突触前或突触后神经调节。的分子特征 受体，信号转导级联被激活，作为一个 结合的结果和激活下游的靶蛋白 也在积极推进中。 酸性钙调蛋白是一种36 kDa的新的 肌动蛋白结合蛋白钙调蛋白的同种型。免疫荧光研究 使用针对其独特的C-末端尾部结构域的肽特异性抗血清， 将蛋白质定位于高膜周转率的区域，特别是， 分裂细胞的分裂沟，神经元的生长锥和位点 伤害。 进一步的研究将致力于阐明其 在伤口愈合和神经再生中的作用。

The following summary describes five projects that attempt to investigate, at the molecular level, the roles of a number of molecules including, kinesin light chains, rab 3, p67 a novel activator of neuronal Cdk5, the modulatory neuropeptides buccalin and myomodulin and acidic calponin, play in neural function. Kinesin light chains, rab 3 and p67 are proteins that either directly or indirectly affect synaptic vesicle transport or fusion. We are currently attempting to address protein function by mutation and knock-out analysis by homologous recombination with kinesin light chains and p67 or by protein expression and microinjection in the case of rab3. We have previously characterized the peptide families of buccalin and myomodulin from the sea hare Aplysia californica and are currently examining how they collectively contribute to pre- or postsynaptic neuromodulation. The molecular characterization of receptors, the signal transduction cascades that are activated as a consequence of binding and the target proteins downstream of activation are also actively being pursued. Acidic calponin is a 36 kDa novel isoform of the actin binding protein calponin. Immunofluorescence studies using peptide specific antisera to its unique C-terminal tail domain have localized the protein to areas of high membrane turnover, particularly, the cleavage furrow of dividing cells, growth cones of neurons and sites of injury. Further research will be directed towards elucidating its role in wound healing and nerve regeneration.