RESEARCH CENTER IN ORAL BIOLOGY

口腔生物学研究中心的总体目标是 研究炎性组织破坏的分子基础和 修理。大量证据表明，细胞因子和生长因子 牙龈局部诱导对微生物定植的反应 调动宿主细胞相互作用的网络，最终导致 组织破坏。这项提议特别针对边界。 免疫反应和组织破坏之间的关系。我们建议使用 多学科的方法，将免疫学家、牙医、 生物化学家和细胞生物学家，并使用最先进的分子 和免疫学方法。该中心由六个相互关联的 研究项目和五个核心。项目8将调查类杆菌 炎症牙龈组织中的内毒素/细胞因子调节；项目4将 研究IgA对细胞炎症反应的调节 5将研究口腔中T细胞/细胞因子对B细胞反应的影响 项目3将研究胶原蛋白的分子机制 口腔粘膜成纤维细胞的降解；项目6将进行 基质金属蛋白酶的功能结构域分析；和项目 7将检查纤维的生物组装和降解 细胞外基质的结构。行政核心意志 为中心提供行政支持和赠款管理专业知识 调查人员。组织采购核心将检索人体组织 中心研究样本，主要取自口腔粘膜。 细胞因子核心将协助调查人员进行细胞因子分析 蛋白质和信使核糖核酸水平。蛋白质核心将提供序列分析 将合成和纯化蛋白质和多肽的寡肽 中心研究项目。DNA Core将为 寡核苷酸合成和DNA领域的研究人员 测序。

The overall objective of the Research Center in Oral Biology is to investigate the molecular basis of inflammatory tissue destruction and repair. A body of evidence suggests that cytokines and growth factors induced locally in the gingiva in response to microbial colonization mobilize a network of host cell interactions that ultimately results in tissue destruction. This proposal specifically targets the boundary between immune response and tissue destruction. We propose to use a multidisciplinary approach which brings together immunologists, dentists, biochemists and cell biologists and employs state-of-the-art molecular and immunologic methods. The Center is composed of six interrelated research projects and five cores. Project 8 will investigate Bacteroides LPS/Cytokine Regulation In Inflamed Gingival Tissue; Project 4 will investigate Regulation of cellular Inflammatory Responses by IgA; Project 5 will investigate T Cells/Cytokines for B Cell Responses in Oral Disease; Project 3 will study the Molecular Mechanisms of Collagen Degradation by Oral Mucosal Fibroblasts; Project 6 will conduct an Analysis of functional domains in Matrix Metalloproteinases; and Project 7 will examine the Biologic Assembly and Degradation of Fibrillar Structures of the Extracellular Matrix. The Administrative Core will provide administrative support and grants management expertise for Center investigators. The Tissue Procurement core will retrieve human tissue samples for Center research, primarily specimens from the oral mucosa. The Cytokine Core will assist investigators with cytokine analyses at the protein and mRNA levels. The Protein core will provide sequence analysis of proteins and peptides and will synthesize and purify oligopeptides for Center research projects. The DNA Core will provide assistance to investigators in the area of oligonucleotide synthesis and DNA sequencing.