STUDIES OF GLOMERULAR CELLS DERIVED FROM TRANSGENIC MICE

肾小球硬化，以肾小球细胞周转增加为特征 而周转增加的细胞外基质周转，是领先的 在美国导致肾衰竭的原因。目前对人类或动物的研究 肾小球硬化(GS)的模型几乎没有关于 细胞和分子的异常在 这种疾病的发生和发展。这在一定程度上是由于 在体内分离和鉴定肾小球的困难，以及事实 也就是说，肾小球包含三种本土细胞类型和一个种群 骨髓来源的巨噬细胞。这是一个特别的问题， 小鼠，我们之所以选择研究这一物种，是因为 关于细胞外基质和生长的大量遗传信息 监管因素。我们已经通过将 单个细胞类型并在体外对其进行表征。这一消息的来源 研究的细胞类型包括正常小鼠、非肥胖糖尿病小鼠和 转基因牛生长激素的小鼠。我们首先研究了这个问题 体外肾小球系膜细胞表型变化的研究 关于这种情况是否发生的问题，时间框架是什么 以及肾小球系膜细胞之间是否存在差异 来自不同分离株或不同来源的细胞。我们在那里发现了 是不同传代的细胞之间的显著差异，以及 在不同的菌株和不同的小鼠品系之间。

Glomerulosclerosis, characterized by increased glomerular cell turnover and increased turnover of extracellular matrix turnover, is the leading cause of renal failure in the US. Current studies in humans or animal models of glomerulosclerosis (GS) have yielded little information about the cellular and molecular abnormalities that are critical in the initiation and progression of this disease. This is due, in part, to the difficulty in isolating and characterizing glomeruli in vivo, and the fact that, the glomerulus contains three indigenous cell types and a population of bone marrow-derived macrophages. This is particularly a problem with mice, a species we have chosen to study because of the availability of considerable genetic information about extracellular matrix and growth regulatory factors. We have approached this problem by isolating the individual cell types and characterizing them in vitro. The sources of the cells types examined include normal mice, non-obese diabetic mice, and mice transgenic for bovine growth hormone. We first examined the question of the phenotypic modulation of glomerular mesangial cells in vitro, addressing the question of whether this occurred, what was the time frame of the occurrence, and whether there was a difference between mesangial cells from different isolates or different sources. We found that there was a marked difference between cells at different passages, as well as between different isolates, and different mouse strains.