MAMMALIAN GENES ENCODING ENHANCER OF SPLIT GROUCHO PROTEIN

小鼠和人编码AES(Split的氨基增强子)和ESG的cDNA (裂解Groucho增强子)蛋白与果蝇有很强的同源性 分离并测序Groucho裂解蛋白增强子。小白鼠 AES-1和AES-2蛋白，可能是由选择性剪接引起的， 分别含有202和196个氨基酸，而小鼠ESG蛋白 由771个氨基酸组成。小鼠和小鼠的氨基酸序列 人类AES蛋白被发现与约50%的 果蝇、小鼠ESG和人的氨基末端区域 TLE(Split的转导素样增强子)蛋白。小鼠俄歇电子能谱 1.5Kb和1.2Kb的转录本在肌肉中大量表达， 心和脑。人的1.6kb和1.4kb的AES转录本 主要存在于肌肉、心脏和胎盘。小鼠ESG 仅发现3.3Kb和4.0Kb的转录本 在睾丸中，4.5Kb的人TLE-1转录本在 肌肉和胎盘与心、脑、肺、肝、肾和 胰腺。人的AES、TLE1和TLE3基因被定位在染色体上 19号、9号和15号，分别使用人类和中国仓鼠杂交 细胞系。

Mouse and human cDNAs encoding AES (Amino Enhancer of Split) and ESG (Enhancer of Split Groucho) proteins with strong homology to Drosophila Enhancer of split groucho protein were isolated and sequenced. Mouse AES-1 and AES-2 proteins, probably resulting from alternative splicing, contain 202 and 196 amino acids, respectively, while mouse ESG protein consists of 771 amino acids. The amino acid sequences of mouse and human AES proteins were found to exhibit approximately 50% identity to the amino-terminal region of Drosophila groucho, mouse ESG and human TLE (Transducin-Like Enhancer of split) proteins. Mouse AES transcripts of 1.5Kb and 1.2Kb were abundantly expressed in muscle, heart and brain. Human AES transcripts of 1.6 Kb and 1.4Kb were predominantly present in muscle, heart and placenta. Mouse ESG (homolog of human TLE 3) transcripts of 3.3Kb and 4.0Kb were found only in testis, while human TLE 1 transcripts of 4.5Kb were more abundant in muscle and placenta compared to heart, brain, lung, liver, kidney and pancreas. Human AES, TLE 1 and TLE 3 genes were mapped to chromosome no. 19, 9 and 15, respectively, using human and Chinese hamster hybrid cell lines.