ANATOMICAL ANALYSIS OF NEURONAL CIRCUITS

该项目旨在提高我们对详细的 丘脑和它的电路。关系到唤醒，光滑- 觉醒周期和皮层下传递的信息 大脑皮层 最近的证据表明， 丘脑网状核的核是至关重要的 产生一些有节奏的振荡。它表征了前脑 活动在慢波圆滑。 本研究的重点是 识别可能影响兴奋性的传入输入 丘脑网状核内的神经元，并确定 这些传入神经与细胞体的关系的细节， 丘脑网状核神经元树突。 在过去的一年中，苍白球的外部投影在 用电子显微镜观察了松鼠猴丘脑网状核 将WGA-HRP注射到外节后的显微镜下 苍白球和GABA包埋后免疫组化 使用胶体金。 最常见的GABA能突触特征 在猴丘脑网状核神经元内有大的终扣 充满了许多多形性囊泡和线粒体。这些 终扣染色密集的GABA，总是突触前，并建立 丘脑体细胞和树突上的对称性突触接触 网状核神经元 少量GABA能谱， 在丘脑内也发现了参与串联突触 网状神经核 GABA染色的轮廓， 参与串联突触的突触较弱，它们含有较少的囊泡 和线粒体比F末端。 外苍白球 用HRP标记的终末，来自有髓鞘的轴突， 类似于F终端;即，它们很大，充满了许多多形性 囊泡和线粒体，总是突触前，染色密集， GABA，并建立对称的突触，经常对胞体和 丘脑网状核神经元近端树突。 详情 这些与已知的其他外源性GABA能输入的关系 进入丘脑网状核有待检查。

This project seeks to improve our understanding of the detailed circuitry of the thalamus and it. relation to arousal, sleek- wakefulness cycles, and information transfer from subcortical relays to the cerebral cortex. Recent evidence suggests that the reticular nucleus of the thalamus reticular nucleus is critical for the generation of some rhythmic oscillation. which characterize forebrain activity during slow-wave sleek. The focus of this study is to identify the afferent inputs which might influence the excitability of neurons within the thalamic reticular nucleus and to determine the details of the relationship of these afferents to the cell bodies and dendrites of thalamic reticular nucleus neurons. During the past year, the external pallidal projection upon the squirrel monkey thalamic reticular nucleus was examined in the electron microscope following injections of WGA-HRP into the external segment of the globus pallidus and post-embedding immunohistochemistry for GABA using colloidal gold. The most common GABAergic synaptic profiles within the monkey thalamic reticular nucleus neuropil are large boutons packed with many pleomorphic vesicles as well as mitochondria. These boutons stain densely for GABA, are-always presynaptic, and establish symmetric synaptic contacts upon the somata and dendrites of thalamic reticular nucleus neurons. A small number of GABAergic profiles which participate in serial synapses was also found within the thalamic reticular nucleus neuropil. The GABA staining within profiles which participate in serial synapses is weak and they contain fewer vesicles and mitochondria than the F terminals. The external pallidal terminals, labeled with HRP, arise from thinly myelinated axons, and resemble F terminals; viz. they are large, packed with many pleomorphic vesicles and mitochondria, are always presynaptic, stain densely for GABA, and establish symmetric synapses frequently upon the somata and proximal dendrites of thalamic reticular nucleus neurons. Details of the relation of these to other extrinsic GABAergic inputs known to enter the thalamic reticular nucleus remain to be examined.