CORE--IMMUNOHISTOCHEMISTRY

组织切片上抗原的免疫组织化学鉴定具有 成为现代组织病理学不可或缺的重要组成部分。 抗原 通过免疫荧光技术或酶- 在包埋或冷冻材料上的基质方法。 虽然这些 方法是常规的软组织标本，免疫组化 骨软骨组织的检查由于 骨和软骨的材料特性。 特殊技术是 需要处理非脱钙骨进行免疫组化， 使用水溶性冷聚合塑料或使用 特殊的粘合剂，支持，并在冷冻薄切片后载玻片 在电动低温恒温器上的材料。 通过这些技术， 存在于细胞表面的抗原，无论是在炎性 软骨、骨、滑膜或任何其他组织中的浸润;以及 非细胞抗原存在于基质中。 的目的 东北俄亥俄州多用途关节炎的免疫组化核心 中心是继续作为一个共享的资源和集中的功能 用于生产与以下相关的单克隆抗体的设施 肌肉骨骼研究和加工，嵌入，切割， 骨、软骨和关节相关的免疫组织化学染色 作为中心正在进行的研究活动的一部分生成的样本 人员的 免疫组化实验室的集中化 提供物资、设备和努力的经济性;提供新的 向目前未使用这些技术的研究者提供技术;以及 促进近地天体运行环境影响评估中心各研究人员之间的互动。

The immunohistochemical identification of antigens on tissue sections has become an integral and valuable part of modern histopathology. Antigens are localized either by immunofluorescent techniques or by enzyme- substrate methods on embedded or frozen materials. Although these methods are routine for soft tissue samples, immunohistochemical examination of osteocartilaginous tissues is made difficult by the material properties of bone and cartilage. Special techniques are required to process nondecalcified bone for immunohistochemistry such as the use of water soluble, cold polymerizing plastics or the use of special adhesives, supports, and slides after thin sectioning of frozen material on a motorized cryostat. With these techniques one can identify antigens present on the surface of cells, whether in inflammatory infiltrates, in cartilage, bone, synovium, or any other tissue; and noncellular antigens present within the matrix. The objective of the Immunohistochemistry Core of the northeast ohio multipurpose Arthritis Center is to continue to function as a shared resource and centralized facility for the production of monoclonal antibodies relevant to musculoskeletal research and for the processing, embedding, cutting, and immunohistochemical staining of bone, cartilage, and joint-related samples generated as part of on-going research activities of center personnel. The centralization of the immunohistochemistry laboratory provides economy of supplies, equipment, and effort; offers new technology to investigators not currently using these techniques; and promotes interactions among the various investigators in the NEOMAC.