REGULATION OF ENERGY METABOLISM IN AGING AND DISEASE--CENTRAL NERVOUS SYSTEM

这个项目研究了线粒体在老年和老年中的功能。 线粒体能量转导减少的病理状态 可能会危及神经元的存活。我们已经研究了这种可能性 线粒体蛋白质合成受损可能导致蛋白质合成减少 老年呼吸链功能。呼吸链复合体 选择用于研究的是其中一个或多个亚基由 MT-DNA；即NADH-CoQ氧化还原酶(复合体1)；细胞色素C氧化酶 (络合物IV)，F1-Fo-ATPase(络合物V)。此外，第三国(ADP-- 依赖)谷氨酸+苹果酸和琥珀酸作为吸收O(2) 对底物进行了研究。在这个时候，已经发现活动是 大脑皮质、海马体和纹状体相似，年轻时(6 与衰老(24mo)雄性大鼠进行比较。在协作中 与LBC的C.Filburn博士一起，我们还启动了一项关于 上述脑区mt-DNA缺失的发生率为 衰老的一种功能。

This project examines mitochondrial functioning in old age and in pathological states in which decreased energy transduction by mitochondria may compromise survival of neurones. We have examined the possibility that impaired mitochondrial protein synthesis may result in decreased respiratory chain function in old age. The respiratory chain complexes chosen for study are those in which one or more subunit is coded for by mT-DNA; viz, NADH-CoQ oxidoreductase (Complex 1); cytochrome c oxidase (Complex IV); F1-Fo-ATPase (Complex V). In addition, State 3 (ADP- dependent) O(2)-uptake with glutamate plus malate and with succinate as substrate was studied. At this time, activities have been found to be similar in cerebral cortex, hippocampus and striatum, when young adult (6 mo) and senescent (24 mo) male rats were compared. In a collaboration with Dr. C. Filburn of LBC, we have also initiated a study of the incidence of deletions of mt-DNA in the brain regions identified above, as a function of aging.