A ROLE FOR INTERLEUKIN 6 IN THE PATHOGENESIS OF HIV INFECTION IN HUMANS

白细胞介素-6（IL-6）是一种多功能细胞因子， 巨噬细胞、成纤维细胞和其它细胞类型。 据了解， 的刺激诱导IL-6分泌在不同的靶细胞， 细胞因子、细菌和细菌产物以及某些病毒。 在 目前的研究，我们发现，艾滋病毒诱导高水平的IL-6分泌， 人单核细胞。 这种效应需要单核细胞/巨噬细胞感染， 因为在热灭活的HIV存在下观察不到。 此外，IL-6的产生首先在单核细胞活化后8-12天检测到。 感染，此时逆转录酶水平开始攀升。 由于HIV在体外诱导IL-6的产生，我们已经检查了 HIV感染者IL-6水平可能升高 个体 正常HIV血清阴性个体的血清水平为 低，B 9试验中测定的平均值为1.6 IL-6 U/ml。 在 与此相反，血清IL-6水平显著升高（p <0.01 在30个HIV血清阳性个体中。 阶段1和2（W.R.分类） 患者（10例研究）的平均IL-6水平为25 U/ml; 3期和4期患者 (10研究）的平均值为46 IL-6 U/ml，5期和6期患者（10 研究）的平均值为27 IL-6 U/ml。 高水平的血清IL-6与 与血清IgG水平直接相关，但与血清 循环中可溶性白细胞介素-2 由于已知IL-6诱导B细胞生长 但不诱导IL-2的产生。 T细胞，这些发现表明体内感染的可能性 HIV诱导单核细胞分泌IL-6。 内源性IL-6 可能是导致HIV感染者IgG水平升高的原因。

Interleukin-6 (IL-6) is a multifunctional cytokine produced in macrophages, fibroblasts and other cell types. It is known that a number of stimuli induce IL-6 secretion in different target cells including cytokines, bacteria and bacterial products and certain viruses. In the present study, we have found that HIV induces high-level IL-6 secretion in human monocytes. This effect requires monocyte/macrophage infection with HIV because it is not observed in the presence of heat-inactivated HIV. In addition, IL-6 production is first detected 8-12 days after monocyte infection, at a time when reverse transcriptase levels begin to climb. Because HIV induces IL-6 production in vitro, we have examined the possibility that IL-6 levels might be elevated in HIV-infected individuals. Serum levels in normal HIV seronegative individuals were low, with a mean of 1.6 IL-6 U/ml as determined in the B9 assay. In contrast, serum IL-6 levels were significantly (p less than 0.01) elevated in 30 HIV seropositive individuals. Stage 1 and 2 (W.R. classification) patients (10 studied) had a mean of 25 IL-6 U/ml; stage 3 and 4 patients (10 studied) had a mean of 46 IL-6 U/ml; and stage 5 and 6 patients (10 studied) had a mean of 27 IL-6 U/ml. High levels of serum IL-6 correlated directly with levels of serum IgG, but did not correlate with serum levels of circulating soluble IL-2. Since IL-6 is known to induce B cell growth and differentiation but not to induce IL-2 production in mitogen-activated T cells, these findings suggest the possibility that infection in vivo with HIV induces IL-6 secretion by monocytes. Endogenously produced IL-6 may be responsible for high IgG levels in HIV-infected individuals.