INTERLEUKIN-10 INHIBITS T CELL PROLIFERATION AND INTERFERON GAMMA PRODUCTION

INTERLEUKIN-10 抑制 T 细胞增殖和干扰素 GAMMA 产生

• 批准号: 3804773
• 负责人: G TOSATO
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3804773
• 关键词: Epstein Barr virus; T lymphocyte; cytokine; human tissue; interferons; interleukin 2; latent virus infection; lymphocyte proliferation; tissue /cell culture; virus infection mechanism

Interleukin-10 (IL-10) was originally identified as a cytokine produced by mouse TH2 cells that inhibits interferon gamma production by TH1 cells. Recently, human IL-10 has been sequenced and found to have approximately 70 % homology with the BCRF-1 open reading frame of Epstein-Barr virus. Similar to mouse IL-10, human IL-10 has been found to inhibit interferon gamma production by human T cells, a property shared by the protein encoded by BCRF-1. The purpose of our investigation was to assess a possible role for IL-10 and BCRF-1 in the control of EBV infection. In particular, we wished to know whether EBV might utilize suppression of Interferon gamma production as a mechanism for establishing latency in normal individuals. A number of experiments have demonstrated that both human IL-10 as well as BCRF-1 protein are potent inhibitors of mitogen-induced T cell proliferation. This inhibitory effect is associated with inhibited IL-2 production and is reversed by addition of exogenous IL-2 to the suppressed cultures. Confirming previous observations, we also found that IL-10 and BCRF-1 protein inhibit Interferon gamma production. This effect, however, appears to be independent of the growth inhibitory functions of IL-10 and BCRF-1.

白介素10(IL-10)最初被认为是一种由 抑制TH1细胞产生干扰素的小鼠TH2细胞。 最近，对人IL-10进行了测序，并发现其具有大约 与Epstein-Barr病毒BCRF-1开放阅读框有70%的同源性。 与小鼠IL-10相似，人IL-10已被发现抑制干扰素 人类T细胞产生伽马，这是蛋白质共有的特性 由BCRF-1编码。我们调查的目的是评估 IL-10和BCRF-1在EB病毒感染控制中的作用在……里面 特别是，我们想知道EBV是否可能利用抑制 干扰素产生作为建立潜伏期的一种机制 普通人。 多项实验表明，人IL-10以及 BCRF-1蛋白是丝裂原诱导的T细胞的有效抑制物 扩散。这种抑制作用与抑制IL-2有关 产生，并通过向被抑制的细胞中添加外源IL-2来逆转 文化。证实了之前的观察，我们还发现IL-10和 BCRF-1蛋白抑制干扰素-γ的产生。然而，这种效果， 似乎独立于IL-10和IL-10的生长抑制功能 BCRF-1。