AIDS-RELATED MYCOBACTERIAL DISEASE--ANTIBODIES TO M. AVIUM IN AIDS SERA

评价发展血清学诊断试验的可行性， 检测MAC感染，我们检查了MAC的体液反应 艾滋病病毒血清阴性和艾滋病患者的疾病。 MAC抗原抗体 用ELISA和免疫印迹法检测了20例AIDS患者血清中的 5例播散性MAC病患者，5例HIV血清阴性患者， 肺MAC感染和20名健康对照。 使用MAC的ELISA研究 声波提取物显示，健康对照和艾滋病 MAC病患者的抗MAC抗体水平相当低， 滴度 来自MAC病患者的HIV-1抗体滴度， 消极的人明显更高。 然而，使用MAC的免疫印迹分析 声波提取物表明，每组都表现出有限的异质性， 回应M。禽抗原 无显著免疫印迹反应性 发现了差异。 用纯化的非结核分枝杆菌抗原进行免疫印迹研究 显示患者血清经常识别重组蛋白， 衍生自19-、27-和35 kDa分枝杆菌抗原。 反19和27 在约50%的MAC患者血清中鉴定出kDa抗体。更大 超过90%的患者血清和仅25%的对照与MK 35反应。 这些 结果最终表明，19-，27-和35 kDa蛋白质是 血清学活性MAC抗原。 此外，尽管声波提取物 不能在患者和对照之间进行充分的血清学区分 血清、重组抗原如MK 35可用作血清学 非结核分枝杆菌病的指标。

To evaluate the feasibility of developing serodiagnostic tests for detecting MAC infections, we have examined the humoral response to MAC disease of HIV-seronegative and AIDS patients. Antibodies to MAC antigens were measured by ELISA and immunoblot analyses in sera from 20 AIDS patients with disseminated MAC disease, 5 HIV-seronegative patients with pulmonary MAC infections, and 20 healthy controls. ELISA studies using MAC sonic extracts demonstrated that the healthy controls and and the AIDS patients with MAC disease had comparable low level anti-MAC antibody titers. The antibody titers from patients with MAC disease who were HIV- negative were significantly higher. However, immunoblot analyses using MAC sonic extracts indicated that each group exhibited a limited heterogeneous response to M. avium antigens. No significant immunoblot reactivity differences were detected. Immunoblot studies using purified nontuberculous mycobacterial antigens revealed that patient sera frequently recognized recombinant proteins derived from 19-, 27-, and 35 kDa mycobacterial antigens. Anti-19 and 27 kDa antibodies were identified in about 50% of MAC patient sera. Greater than 90% of patient sera and only 25% of controls reacted with MK35. These results conclusively demonstrate that the 19-, 27-, and 35 kDa proteins are serologically active MAC antigens. In addition, although sonic extracts do not permit adequate serological discrimination between patient and control sera, recombinant antigens such as MK35 may be useful as serological indicators of nontuberculous mycobacterial disease.