CHARACTERIZATION OF MYCOBACTERIUM INTRACELLULARE LAMBDA-GT11 RECOMBINANT CLONES

在过去的一年里，免疫学实验室评估了 三株胞内分枝杆菌的生化和免疫学特性 抗原，并已完成编码基因的核苷酸序列 这些抗原。 MI22-核苷酸序列分析表明，MI22是 具有血清活性的19 kDa结核分枝杆菌蛋白。纯化的MI22是 70%的结核病患者血清和45%的结核病患者血清可以识别 艾滋病患者伴发MAC病。遗传和生化特征 提示MI22是一种脂蛋白。 MI43-核苷酸序列分析表明，MI43基因编码一个 27 kDa蛋白。血清学检测表明，90%的血清来自 结核病患者和50%的艾滋病患者血清与该蛋白发生反应。 遗传和生化分析表明，MI43是一种脂蛋白。 MI85基因序列与已知基因的计算机比对 序列分析表明，MI85与MI85的蛋白质序列同源性为70% 大肠杆菌过氧化氢酶及其60%的蛋白质序列同源性 一种沙门氏菌过氧化氢酶。此外，含有下列物质的大肠杆菌裂解物 过表达的MI85蛋白具有显著的过氧化物酶和过氧化氢酶活性 酶活性。这些遗传和生化结果表明 MI85编码过氧化氢酶，这是一种对 分枝杆菌在吞噬体内的存活。

During the past year, the Laboratory of Immunology has evaluated the biochemical and immunological properties of three M. intracellulare antigens and has completed the nucleotide sequences of the genes encoding these antigens. MI22- Nucleotide sequence analysis demonstrated that MI22 is a homolog of the serologically active 19 kDa M. tuberculosis protein. Purified MI22 is recognized by 70% of sera from tuberculosis patients and 45% of sera from AIDS patients with MAC disease. Genetic and biochemical characterization suggest that MI22 is a lipoprotein. MI43- Nucleotide sequence analysis indicates that the MI43 gene encodes a 27 kDa protein. Serological assays have demonstrated that 90% of sera from tuberculosis patients and 50% of AIDS patient sera react with this protein. Genetic and biochemical analyses suggest that MI43 is a lipoprotein. MI85- Computer-based comparisons of the MI85 gene sequence to known gene sequences demonstrated that MI85 has 70% protein sequence identity to the an Escherichia coli peroxidase-catalase and 60% protein sequence identity to a Salmonella catalase. In addition, E. coli lysates containing overexpressed MI85 protein exhibited significant peroxidase and catalase enzymatic activity. These genetic and biochemical results indicate that the MI85 encodes a peroxidase-catalase, an enzyme which is critical for the intraphagosomal survival of mycobacteria.