PREVALENCE OF YERSINIA ENTEROCOLITICA IN STORED RED BLOOD CELLS

对储存红血的过时冰箱单元进行的大型生物负载调查 细胞（RBC）由Upjohn公司在1989-1991年期间进行，其中 共检测了11，702个红细胞样本池，代表585，100个单位， 生物负荷使用标准倾注平板培养方法，旨在检测 细菌和真菌污染物的一般谱。 在70个阳性中， 分离培养物，没有鉴定为Y。小肠结肠炎或任何相关 物种 为了确定所用方法的灵敏度， 特异性检测Y.小肠结肠炎，进行了验证研究 使用直接从Upjohn获得的RBC池的存储样本。 二十 RBC池样品分别接种105、104和103个微生物/ml 从Y.小肠结肠炎菌在全血中生长。 接种的 通过Upjohn调查中使用的相同方法培养池。 所有接种的合并液均产生了基于 已知输入接种物。 结果表明，Y. Upjohn研究中调查的任何红细胞池中的小肠结肠炎菌 被发现了 这些数据证实了红细胞污染的罕见性 通过Y。小肠结肠炎，并促成了血液制品的决定 咨询委员会不建议减少冰箱储存的日期 红细胞

A large bioburden survey of outdated units of refrigerator stored red blood cells (rbc) was performed by the Upjohn Company during 1989-1991, in which a total of 11,702 rbc pools, representing 585,100 units, were tested for bioburden using a standard pour plate culture method designed to detect a general spectrum of bacterial and fungal contaminants. Of the 70 positive cultures isolated, none were identified as Y. enterocolitica or any related species. To determine the sensitivity of the method employed for the specific detection of Y. enterocolitica, a validation study was carried out using stored samples of rbc pools obtained directly from Upjohn. Twenty samples of rbc pools were inoculated with 105 104 and 103 organisms per ml from a culture of Y. enterocolitica grown in whole blood. The inoculated pools were cultured by the identical method employed in the Upjohn survey. All inoculated pools yielded the predicted colony counts based upon the known input inoculum. It was concluded that the presence of Y. enterocolitica in any of the rbc pools surveyed in the Upjohn study would have been detected. These data confirmed the rarity of rbc contamination by Y. enterocolitica, and contributed to a decision by the Blood Products Advisory Committee not to recommend reduced dating for refrigerator stored RBC's.