MODE OF ACTION OF REPRODUCTIVE HORMONES

该中心的长期目标是研究 与生殖相关的类固醇激素和糖蛋白激素的作用 流程。来年的目标包括：(1)净化核 鸡输卵管染色质中孕酮受体蛋白； 制备和检测抗核受体蛋白的单抗； 用物理化学方法研究受体蛋白DNA相互作用； 描述和比较鸡输卵管受体的各种形式； (5)描述各种形式的亚基的特征 孕激素受体；(6)研制抗孕酮受体的单抗 孕激素受体的各种形式；(7)确定孕激素受体的来源 孕激素受体异质性；(8)研究碳水化合物链是如何 人绒毛膜促性腺激素分子对卵巢腺苷环化酶的影响(9) 从黄体细胞膜中分离出一种58K蛋白水解酶；(10)纯化制备 抗hCG受体的单抗；及(11)研究hCG 使用荧光偏振技术的受体相互作用。

The long-term goals of this center are to investigate the mechanisms of action of steroid and glycoprotein hormones related to the reproductive processes. The aims for the coming year include: (1) purify the nuclear progesterone-receptor acceptor protein from chick oviduct chromatin; (2) prepare and test monoclonal antibodies to nuclear acceptor protein; (3) study acceptor protein DNA interactions using physical-chemical means; (4) characterize and compare the various forms of the chick oviduct receptor; (5) characterize the individual subunits of the various forms of the progesterone receptor; (6) develop monoclonal antibodies against the various forms of the progesterone receptor; (7) identify the origin of progesterone receptor heterogeneity; (8) study how the carbohydrate chains on the hCG molecule effect the ovarian adenylate cyclase enzyme; (9) isolate a 58k protease from luteal cell membranes; (10) purify and prepare monoclonal antibodies against the hCG receptor; and (11) study hCG receptor interactions using fluorescent polarization techniques.