Characterization of a reproductive hormone receptor from mosquitoes

蚊子生殖激素受体的表征

• 批准号: 8649425
• 负责人: Kevin James Vogel
• 金额: $ 5.33万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-03-01 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8649425
• 关键词: Adult; Aedes; Affect; Affinity; Amino Acids; Animals; Antibodies; Area; Arthropod Vectors; Binding; Biological Assay; Biology; Blood; Blood Proteins; Brain; Cell Line; Complement; Culicidae; Data; Dengue; Deposition; Development; Digestion; Diptera; Disease; Disease Vectors; Doctor of Philosophy; Ecdysteroids; Egg Yolk Proteins; Endocrinology; Event; Family; Family member; Fat Body; Female; Genes; Genomics; Goals; Hormonal; Hormone Receptor; Hormones; Human; Immunoblotting; Insect Vectors; Insecta; Insulin; Insulin Receptor; Interruption; Malaria; Mediating; Membrane; Methods; Midgut; Mosquito Control; Names; Neuropeptides; Nutrient; Oocytes; Oogenesis; Outcome; Ovary; Pattern; Peptide Hydrolases; Peptides; Physiological; Physiological Processes; Preparation; Prevalence; Process; Property; Protein Biosynthesis; Proteins; RNA; RNA Interference; Radiolabeled; Receptor Protein-Tyrosine Kinases; Receptor Signaling; Regulation; Reproduction; Reproductive Endocrinology; Reproductive Physiology; Reproductive Process; Research; Role; Signal Pathway; Signal Transduction; Surface; Testing; Tissues; Training; Virus; Vitellogenins; Work; absorption; comparative genomics; deprivation; disease transmission; egg; feeding; fly; human disease; insight; interest; novel; pathogen; public health relevance; radiotracer; receptor binding; receptor expression; reproductive hormone; research study; response; spatiotemporal; transmission process; uptake; vector

DESCRIPTION (provided by applicant): Reproduction in mosquitoes, like all animals, is a tightly regulated process that involves coordination of several physiological events including nutrient uptake and egg maturation. The neuropeptide ovary ecdysteroidogenic hormone (OEH) - released from the brain after a bloodmeal - has been demonstrated to be a master regulator of reproduction in mosquitoes. In concert with insulin-like peptides (ILPs), OEH stimulates the release of ecdysteroids from the mosquito ovaries, which then stimulate events leading to the maturation of an egg clutch. Deprivation of OEH from bloodfed mosquitoes completely arrests reproduction, and is a promising target for disruption strategies. Unlike ILPs, OEH lacks a characterized receptor and the signaling pathway triggered by OEH and leading to ecdysteroid release is unknown. Utilizing a comparative genomics and functional approach, I have identified a candidate OEH receptor in Aedes aegypti named R1915. To elucidate the role of R1915 in OEH signaling, I propose the following 3 aims: 1) biochemically confirm R1915 is the OEH receptor and fully assess spatiotemporal expression of the receptor, 2) characterize the signaling pathway of OEH, and 3) assess how OEH and R1915 influence reproductive processes including bloodmeal digestion and ovary maturation. The results of this research will provide insights into the regulation of reproduction in mosquitoes, providing new targets for strategies to disrupt mosquito-vectored disease transmission.

描述（由申请人提供）：与所有动物一样，蚊子的繁殖是一个受到严格调控的过程，涉及多种生理事件的协调，包括营养吸收和卵成熟。神经肽卵巢蜕皮类固醇激素（OEH）——吸血后从大脑中释放出来——已被证明是蚊子繁殖的主要调节因子。 OEH 与类胰岛素肽 (ILP) 协同作用，刺激蚊子卵巢释放蜕皮类固醇，然后刺激导致卵成熟的事件。剥夺吸血蚊子的 OEH 会完全阻止其繁殖，并且是破坏策略的一个有希望的目标。与 ILP 不同，OEH 缺乏特征受体，并且由 OEH 触发并导致蜕皮类固醇释放的信号通路尚不清楚。利用比较基因组学和功能方法，我在埃及伊蚊中鉴定出了一种候选 OEH 受体，名为 R1915。为了阐明R1915在OEH信号传导中的作用，我提出以下3个目标：1）生化确认R1915是OEH受体并全面评估受体的时空表达，2）表征OEH信号传导通路，3）评估OEH和R1915如何影响生殖过程，包括血粉消化和卵巢成熟。这项研究的结果将为蚊子的繁殖调节提供见解，为破坏蚊子传播疾病传播的策略提供新的目标。