T CELL COSTIMULATION BY INTERLEUKIN-1 AND INTERLEUKIN-6

两种单核细胞衍生的细胞因子，白细胞介素-1（IL-1）和白细胞介素-6 已报道共刺激单核细胞耗竭的T细胞 在有丝分裂原的存在下，这种效应已被归因于 这些分子的附属功能。我们现在进一步研究了 IL-1 + IL-6的辅助功能潜力，并检查这些 细胞因子促进T细胞与有丝分裂原的生长。总之，IL-1和IL-6 在很小程度上，协同促进 高纯度人外周血T细胞的增殖， 植物血凝素（PHA）。然而，IL-1加IL-6的最大共刺激 在很宽的浓度范围内， 由最佳数量的单核细胞诱导。此外，与单核细胞相比， 共同刺激同样有效的T4阳性和T8阳性细胞， IL-1 + IL-6共刺激T4阳性淋巴细胞明显优于IL-1 + IL-6。 T8阳性细胞。IL-1加IL-6诱导T细胞分泌IL-2 用PHA、IL-2受体抗体和抗Tac共刺激， 显著抑制IL-1和IL-6共刺激的PHA激活的T细胞。在 此外，IL-1加IL-6增强表面IL-2受体的表达。 因为IL-1加IL-6的共刺激作用是定量的， 比单核细胞的小，并且优先导向T4 与T8阳性T细胞相反，IL-1加IL-6，一起， 似乎代表一组选择性的单核细胞衍生的辅助信号。

Two monocyte-derived cytokines, Interleukin-1 (IL-1) and Interleukin-6 (IL-6); have been reported to costimulate monocyte-depleted T cell populations in the presence of mitogen, and this effect has been attributed to an accessory function of these molecules. We have now examined further the accessory function potential of IL-1 plus IL-6, and examined how these cytokines promote T cell growth with mitogen. Together, IL-1 and IL-6 additively and, to a small degree, synergistically promote the proliferation of highly purified human peripheral blood T cells with phytohemagglutinin (PHA). However, maximum costimulation by IL-1 plus IL-6 over a wide range of concentrations is significantly smaller than that induced by optimal numbers of monocytes. Also, in contrast to monocytes that costimulate equally effectively T4 positive and T8 positive cells, IL-1 plus IL-6 costimulate T4 positive lymphocytes in marked preference to T8 positive cells. IL-1 plus IL-6 induces IL-2 secretion in T cell cultures costimulated with PHA, and an antibody to the IL-2 receptor, and anti-Tac, markedly inhibits PHA-activated T cells costimulated by IL-1 plus IL6. In addition, IL-1 plus IL-6 enhance the expression of surface IL-2 receptors. Because the costimulatory effect of IL-1 plus IL-6 is quantitatively smaller than that of monocytes, and it is preferentially directed toward T4 positive as opposed to T8 positive T cells, IL-1 plus IL-6, together, appear to represent a selective set of monocyte-derived accessory signals.