INFECTION OF RABBITS WITH HIV-1

动物模型是疫苗开发的一个重要方面。黑猩猩可以 感染HIV-1，但不会发展成艾滋病。二是 可供研究的黑猩猩总数很少。最近， 兔子已经被证明感染了HIV-1。兔子可以提供 艾滋病早期安全性和毒性试验的有用模型 疫苗，并可能提供一些免疫原性的指示，为一些 还有潜在的免疫原我们给每只兔子接种了 以下HIV-1毒株：IIIB、RF、Mn、Zaire和BR。我们看了 通过蛋白质印迹和ELISA分析血清来发展感染，以及 培养淋巴细胞并监测培养物的逆转录酶 和HIV-1抗原的产生。还检测了淋巴细胞的前病毒 使用聚合酶链反应（PCR）。尸检时，大脑， 淋巴细胞、淋巴结、圆小囊、脾脏和胸腺， 收集用于培养和通过PCR进行测试。另外这些 组织进行组织病理学检查。 我们的结果表明，5株HIV-1感染兔， 与接种物滴度无关。只有菌株RF和Mn诱导 可检测的抗包膜抗体。在检测的组织中， 和淋巴结病毒常呈阳性。没有一只兔子 免疫缺陷的迹象，检查的组织正常 组织学上在HIV-1毒株之间观察到的差异 兔可能产生这些不同的HIV-1发病机制的重要模型 菌株

Animal models are an important aspect vaccine development. Chimpanzees can be infected with HIV-1, however they do not develop AIDS. Secondly, the total number of Chimpanzees available for research is small. Recently, rabbits have been show to become infected with HIV-1. Rabbits could provide a useful model for early safety and toxicity tests of candidate AIDS vaccines and may provide some indication of immunogenicity for some of the potential immunogens, as well. We inoculated one rabbit each with one of the following HIV-1 strains: IIIB, RF, Mn, Zaire, and BR. We looked at the development of infection by analyzing sera by Western blot and ELISA, and culturing lymphocytes and monitoring the cultures for reverse transcriptase and HIV-1 antigen production. Lymphocytes were also tested for provirus using the polymerase chain reaction (PCR). At necropsy, the brain, lymphocytes, lymph node, sacculus rotundus, spleen, and thymus were collected for culture and for testing by the PCR. In addition, these tissues were examined histopathologically. Our results indicate that the five strains of HIV-1 infected rabbits variably, unrelated to inoculum titer. Only the strains RF and Mn induced detectable antienvelope antibodies. Of the tissues tested the lymphocytes and lymph node were often positive for virus. None of the rabbits showed signs of immune deficiency, and the tissues examined were normal histologically. The differences observed between the HIV-1 strains in rabbits may yield important models of pathogenesis of these different HIV-1 strains.