EXPRESSION OF TRANSFORMING GROWTH FACTOR-B HUMAN NEURAL CELL LINES

尽管人们对转化生长的生理作用知之甚少 体内转化生长因子-b和发育中小鼠的免疫组织化学研究 胚胎与转化生长因子-β参与肿瘤细胞的增殖和分化 一般的组织，特别是间充质组织(骨、肌肉和血液 血管)和神经沟来源的血管。有趣的是，激烈的 在起源于神经脊的颅面间充质中发现了染色， 注定要变成下颌骨、上颌骨、上颌骨、上颌骨、鼻和鼻窦。 我们最近已经获得了所有三种转化生长因子-β亚型(1，2和3)的cdna探针。 来自美国国立卫生研究院的Anita Roberts博士，抗转化生长因子-β1和2的抗体是 在商业上可以买到。我们实验室里还有12个人类肿瘤细胞 LINE起源于各种神经肿瘤，从传统的 神经母细胞瘤，外周原始神经外胚层肿瘤，嗅觉 神经母细胞瘤。 我们将研究转化生长因子-β1、转化生长因子-2和转化生长因子-3在这些神经细胞中的表达。 通过Northern和Western blotting在mRNA和蛋白质水平克隆，以及 免疫荧光免疫细胞化学染色。各式各样的 可以观察到这三种转化生长因子-b亚型的表达，暗示着 这种生长因子存在于神经性儿童肿瘤中。此外，还有几个 可能出现的表达方式可能会进一步证实我们的怀疑 在这些肿瘤的发展中不同的致病途径，以及 最后可作为鉴别诊断的标记物。

Although little is known about the physiologic role of Transforming Growth Factor (TGF)-b in vivo, immunohistochemical studies in the developing mouse embryo have implicated TGF-b in proliferation and differentiation of tissues in general especially the mesenchymally (bone, muscle, and blood vessels) and neural crest derived ones. Interestingly enough, intense staining is found in the craniofacial mesenchyme of neural crest origin, destined to become mandible, maxilla, palate, nose, and nasal sinuses. We have recently obtained cDNA probes for all 3 TGF-b subtypes (1,2 and 3) from Dr. Anita Roberts (NIH), and antibodies against the TGF-b1 and 2 are available commercially. We also have in our laboratory 12 human tumor cell lines originated from various neural tumors, ranging from conventional neuroblastoma, to peripheral primitive neuroectodermal tumors, to olfactory neuroblastoma. We will study the expression of TGF-b1, 2, and 3 in all these neural cell lines at the mRNA and protein level by Northern and Western blotting, and immunocytochemical staining by immunofluorescence. Various patterns of expression of these 3 TGF-b subtypes may be observed implicating a role of this growth factor in the neural childhood tumors. In addition, several possible emerging patterns of expression may further confirm our suspicion of different pathogenetic pathways in the development of these tumors, and finally may serve as differential markers in their diagnosis.